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Literature summary for 1.4.1.1 extracted from

  • Ye, W.; Huo, G.; Chen, J.; Liu, F.; Yin, J.; Yang, L.; Ma, X.
    Heterologous expression of the Bacillus subtilis (natto) alanine dehydrogenase in Escherichia coli and Lactococcus lactis (2009), Microbiol. Res., 165, 268-275.
    View publication on PubMed

Application

Application Comment Organism
nutrition Lactococcus lactis strain NZ9000 expressing the enzyme from Bacillus subtilis can be used in imporvement of dairy fermentation for developing healthy yogurts with sweet taste or other fermented dairy foods Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
gene ald, functional heterologous expression in Escherichia coli strain JM109, as GST-tagged protein, and in Lactococcus lactis strain NZ9000 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, recombinant GST-tagged enzyme, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + NH3 + NADH + H+ Bacillus subtilis
-
L-alanine + H2O + NAD+
-
?
pyruvate + NH3 + NADH + H+ Bacillus subtilis natto
-
L-alanine + H2O + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene ald
-
Bacillus subtilis natto
-
gene ald
-
no activity in Lactococcus lactis
-
strain NZ9000
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chrmatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NH3 + NADH + H+
-
Bacillus subtilis L-alanine + H2O + NAD+
-
?
pyruvate + NH3 + NADH + H+
-
Bacillus subtilis natto L-alanine + H2O + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 70000, recombinant GST-tagged enzyme, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
ALADH
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 42 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Bacillus subtilis