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Literature summary for 1.4.1.1 extracted from

  • Giffin, M.M.; Modesti, L.; Raab, R.W.; Wayne, L.G.; Sohaskey, C.D.
    ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase (2012), J. Bacteriol., 194, 1045-1054.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information generation of ald knockout strain RVW7 from wild-type strain H37Rv Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of ald and glyoxylate dehydrogenae activities, overview Mycobacterium tuberculosis
0.0384
-
NADH pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
0.1328
-
NAD+ pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
2.3
-
NH3 pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
2.8
-
pyruvate pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
4.3
-
L-alanine pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol minor fraction Mycobacterium tuberculosis 5829
-
membrane major fraction Mycobacterium tuberculosis 16020
-
additional information no enzyme detected in the extracellular medium Mycobacterium tuberculosis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + H2O + NAD+ Mycobacterium tuberculosis
-
pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ Mycobacterium tuberculosis H37Rv
-
pyruvate + NH3 + NADH + H+
-
r
additional information Mycobacterium tuberculosis enzyme Ald has both pyruvate and glyoxylate aminating activities ?
-
?
additional information Mycobacterium tuberculosis H37Rv enzyme Ald has both pyruvate and glyoxylate aminating activities ?
-
?
pyruvate + NH3 + NADH + H+ Mycobacterium tuberculosis
-
L-alanine + H2O + NAD+
-
r
pyruvate + NH3 + NADH + H+ Mycobacterium tuberculosis H37Rv
-
L-alanine + H2O + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
gene ald
-
Mycobacterium tuberculosis H37Rv
-
gene ald
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities in different strains, overview Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + NAD+
-
Mycobacterium tuberculosis pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+
-
Mycobacterium tuberculosis H37Rv pyruvate + NH3 + NADH + H+
-
r
additional information enzyme Ald has both pyruvate and glyoxylate aminating activities Mycobacterium tuberculosis ?
-
?
additional information enzyme Ald has both pyruvate and glyoxylate aminating activities Mycobacterium tuberculosis H37Rv ?
-
?
pyruvate + NH3 + NADH + H+
-
Mycobacterium tuberculosis L-alanine + H2O + NAD+
-
r
pyruvate + NH3 + NADH + H+
-
Mycobacterium tuberculosis H37Rv L-alanine + H2O + NAD+
-
r

Synonyms

Synonyms Comment Organism
ALD
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
ALD activity Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mycobacterium tuberculosis
NADH
-
Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis transcription of ald is induced when alanine is the sole nitrogen source up

General Information

General Information Comment Organism
malfunction an ald knockout strain grows without alanine or glycine and is able to utilize glycine but not alanine as a nitrogen source Mycobacterium tuberculosis
physiological function enzyme Ald may have several functions, including ammonium incorporation and alanine breakdown. Ald plays an essential role in the utilization of alanine but not of glycine. Ald is not essential for the breakdown of glycine Mycobacterium tuberculosis