Application | Comment | Organism |
---|---|---|
analysis | an enzymatic assay system to eliminate or measure D-Ala, which is reported to affect the taste of seafoods or sake, is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). Using the assay system, the D-Ala contents of 7 crustaceans, i. e. mosa shrimp (Argis lar, Mosaebi), spiny lebbeid (Lebbeus groenlandicus, Oniebi), deepwater prawn (Pandalus eous,Amaebi), flathead lobster (Ibacus ciliates, Uchiwaebi), snow crab (Chionoecetes opilio, Zuwaigani), red snow crab (Chionoecetes japonicus, Benizuwaigani), and horsehair crab (Erimacrus isenbeckii, Kegani), are determined | Phormidium lapideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an enzymatic assay system to eliminate or measure D-Ala, which is reported to affect the taste of seafoods or sake, is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. NADH nonenzymatically reduces WST-1 to form water soluble formazan. Using the assay system, the D-Ala contents of 7 crustaceans are determined. Method evaluation, overview | Phormidium lapideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | Phormidium lapideum | - |
pyruvate + NH3 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormidium lapideum | O52942 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | - |
Phormidium lapideum | pyruvate + NH3 + NADH + H+ | - |
? | |
additional information | an enzymatic assay system to eliminate or measure D-Ala is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. NADH nonenzymatically reduces WST-1 to form water soluble formazan.Using the assay system, the D-Ala contents of 7 crustaceans are determined. Method evaluation, overview | Phormidium lapideum | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Phormidium lapideum |
L-AlaDH | - |
Phormidium lapideum |
L-alanine dehydrogenase | - |
Phormidium lapideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phormidium lapideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Phormidium lapideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Phormidium lapideum |