Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.1 extracted from

  • Ashida, H.; Sawa, Y.; Yoshimura, T.
    Enzymatic determination of D-alanine with L-alanine dehydrogenase and alanine racemase (2021), Biosci. Biotechnol. Biochem., 85, 2221-2223 .
    View publication on PubMed

Application

Application Comment Organism
analysis an enzymatic assay system to eliminate or measure D-Ala, which is reported to affect the taste of seafoods or sake, is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). Using the assay system, the D-Ala contents of 7 crustaceans, i. e. mosa shrimp (Argis lar, Mosaebi), spiny lebbeid (Lebbeus groenlandicus, Oniebi), deepwater prawn (Pandalus eous,Amaebi), flathead lobster (Ibacus ciliates, Uchiwaebi), snow crab (Chionoecetes opilio, Zuwaigani), red snow crab (Chionoecetes japonicus, Benizuwaigani), and horsehair crab (Erimacrus isenbeckii, Kegani), are determined Phormidium lapideum

Protein Variants

Protein Variants Comment Organism
additional information an enzymatic assay system to eliminate or measure D-Ala, which is reported to affect the taste of seafoods or sake, is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. NADH nonenzymatically reduces WST-1 to form water soluble formazan. Using the assay system, the D-Ala contents of 7 crustaceans are determined. Method evaluation, overview Phormidium lapideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + H2O + NAD+ Phormidium lapideum
-
pyruvate + NH3 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Phormidium lapideum O52942
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + NAD+
-
Phormidium lapideum pyruvate + NH3 + NADH + H+
-
?
additional information an enzymatic assay system to eliminate or measure D-Ala is constructed using alanine racemase (L-AlaR from Synechocystis sp. PCC6803) and L-alanine dehydrogenase (L-AlaDH from Phormidium lapideum). D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. NADH nonenzymatically reduces WST-1 to form water soluble formazan.Using the assay system, the D-Ala contents of 7 crustaceans are determined. Method evaluation, overview Phormidium lapideum ?
-
-

Synonyms

Synonyms Comment Organism
ALD
-
Phormidium lapideum
L-AlaDH
-
Phormidium lapideum
L-alanine dehydrogenase
-
Phormidium lapideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Phormidium lapideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Phormidium lapideum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Phormidium lapideum