Cloned (Comment) | Organism |
---|---|
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of ald knockout strain RVW7 from wild-type strain H37Rv | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
glyoxylate | pH 8.5, 22°C, recombinant enzyme | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | minor fraction | Mycobacterium tuberculosis | 5829 | - |
membrane | major fraction | Mycobacterium tuberculosis | 16020 | - |
additional information | no enzyme detected in the extracellular medium | Mycobacterium tuberculosis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene ald | - |
Mycobacterium tuberculosis H37Rv | - |
gene ald | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NH3 + NADH + H+ | - |
Mycobacterium tuberculosis | glycine + H2O + NAD+ | - |
ir | |
glyoxylate + NH3 + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | glycine + H2O + NAD+ | - |
ir | |
additional information | the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |
Organism | Comment | Expression |
---|---|---|
Mycobacterium tuberculosis | transcription of ald is induced when alanine is the sole nitrogen source | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays an essential role in the utilization of alanine, but not of glycine, as a nitrogen source | Mycobacterium tuberculosis |