BRENDA - Enzyme Database
show all sequences of 1.4.1.12

A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine

Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.; J. Bacteriol. 191, 3162-3167 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-amino-4-ketopentanoate
70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate
Acetoanaerobium sticklandii
acetyl-CoA
30% inhibition in the presence of 1 mM acetyl-CoA
Acetoanaerobium sticklandii
D-alanine
45% inhibition in the presence of 1 mM D-alanine
Acetoanaerobium sticklandii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
NADP+
-
Acetoanaerobium sticklandii
2100
-
NAD+
-
Acetoanaerobium sticklandii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
SDS-PAGE
Acetoanaerobium sticklandii
38930
-
calculated from cDNA
Acetoanaerobium sticklandii
Organism
Organism
UniProt
Commentary
Textmining
Acetoanaerobium sticklandii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4-diaminopentanoate + H2O + NAD+
enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)
704292
Acetoanaerobium sticklandii
2-amino-4-oxopentanoate + NH3 + NADH
-
-
-
?
2,4-diaminopentanoate + H2O + NADP+
enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)
704292
Acetoanaerobium sticklandii
2-amino-4-oxopentanoate + NH3 + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
gel filtration, 2 * 38000 Da
Acetoanaerobium sticklandii
Synonyms
Synonyms
Commentary
Organism
2R,4S-2,4-diaminopentanoate dehydrogenase
-
Acetoanaerobium sticklandii
DAPDH
-
Acetoanaerobium sticklandii
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay carried out at room temperature
Acetoanaerobium sticklandii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5
-
NADP+
-
Acetoanaerobium sticklandii
34
-
NAD+
-
Acetoanaerobium sticklandii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Acetoanaerobium sticklandii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+
Acetoanaerobium sticklandii
NADP+
the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+
Acetoanaerobium sticklandii
pyridoxal 5'-phosphate
-
Acetoanaerobium sticklandii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+
Acetoanaerobium sticklandii
NADP+
the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+
Acetoanaerobium sticklandii
pyridoxal 5'-phosphate
-
Acetoanaerobium sticklandii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-amino-4-ketopentanoate
70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate
Acetoanaerobium sticklandii
acetyl-CoA
30% inhibition in the presence of 1 mM acetyl-CoA
Acetoanaerobium sticklandii
D-alanine
45% inhibition in the presence of 1 mM D-alanine
Acetoanaerobium sticklandii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
NADP+
-
Acetoanaerobium sticklandii
2100
-
NAD+
-
Acetoanaerobium sticklandii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
SDS-PAGE
Acetoanaerobium sticklandii
38930
-
calculated from cDNA
Acetoanaerobium sticklandii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4-diaminopentanoate + H2O + NAD+
enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)
704292
Acetoanaerobium sticklandii
2-amino-4-oxopentanoate + NH3 + NADH
-
-
-
?
2,4-diaminopentanoate + H2O + NADP+
enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)
704292
Acetoanaerobium sticklandii
2-amino-4-oxopentanoate + NH3 + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
gel filtration, 2 * 38000 Da
Acetoanaerobium sticklandii
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay carried out at room temperature
Acetoanaerobium sticklandii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5
-
NADP+
-
Acetoanaerobium sticklandii
34
-
NAD+
-
Acetoanaerobium sticklandii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Acetoanaerobium sticklandii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.016
-
NAD+
-
Acetoanaerobium sticklandii
2.56
-
NADP+
-
Acetoanaerobium sticklandii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.016
-
NAD+
-
Acetoanaerobium sticklandii
2.56
-
NADP+
-
Acetoanaerobium sticklandii
Other publictions for EC 1.4.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742928
Fukuyama
Characterization of a thermos ...
Fervidobacterium nodosum
J. Biosci. Bioeng.
117
551-556
2014
-
-
1
-
-
-
10
4
-
1
-
2
-
9
-
-
-
-
-
-
-
-
3
1
1
1
-
1
4
1
1
-
2
1
-
-
-
-
1
2
-
-
-
-
10
1
4
-
1
-
2
-
-
-
-
-
-
-
-
3
1
1
-
1
4
1
1
-
-
-
1
1
-
4
4
704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
1
2
1
-
-
2
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
-
-
-
2
1
1
-
-
2
1
-
-
-
-
-
-
-
2
2
391435
Somack
2,4-Diaminopentanoic acid C4 d ...
Acetoanaerobium sticklandii
J. Biol. Chem.
247
385-388
1973
-
-
-
-
-
-
4
-
-
-
4
1
-
1
-
-
1
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
4
1
-
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391436
Stadtman
-
Lysine metyabolim by clostridi ...
Clostridium sp., Acetoanaerobium sticklandii, Clostridium sp. M-E SB4
Adv. Enzymol. Relat. Areas Mol. Biol.
38
441-445
1973
-
-
-
-
-
-
3
4
-
-
1
6
-
3
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
-
-
3
-
4
-
-
1
6
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
391437
Tsuda
Ornithine metabolism by Clostr ...
Acetoanaerobium sticklandii
J. Biol. Chem.
245
5914-5926
1970
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
1
-
-
-
1
1
2
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-