Literature summary for 1.4.1.13 extracted from

Dincturk, H.; Cunin, R.; Akce, H.
Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii (2011), Microbiol. Res., 166, 294-303 .

Search for more literature for 1.4.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
a construct with the His-tag extension is cloned and expressed in Escherichia coli as soluble protein	Pyrococcus horikoshii
expressed in native form (GLTY1) as well as with an added His-tag (GLTY2). A construct with the His-tag extension is cloned and expressed in Escherichia coli as soluble protein	Pyrococcus horikoshii OT3

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O58606	-	-
Pyrococcus horikoshii OT-3	O58606	-	-
Pyrococcus horikoshii OT3	O59547	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+	NADPH-dependent oxidoreductase activity using artificial electron acceptors iodonitrotetrazolium chloride at thermophilic conditions	Pyrococcus horikoshii	L-glutamate + NADP+	-	?
L-glutamine + 2-oxoglutarate + NADPH + H+	NADPH-dependent oxidoreductase activity using artificial electron acceptors iodonitrotetrazolium chloride at thermophilic conditions	Pyrococcus horikoshii OT3	L-glutamate + NADP+	-	?
L-glutamine + 2-oxoglutarate + NADPH + H+	NADPH-dependent oxidoreductase activity using artificial electron acceptors iodonitrotetrazolium chloride at thermophilic conditions	Pyrococcus horikoshii OT-3	L-glutamate + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 52400, calculated from sequence	Pyrococcus horikoshii
?	x * 51900, calculated from sequence	Pyrococcus horikoshii OT3

Synonyms

Synonyms	Comment	Organism
PH0876	-	Pyrococcus horikoshii
PH1873	-	Pyrococcus horikoshii OT3

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	enzyme extract is clearly very stable	Pyrococcus horikoshii

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Release 2023.1 (January 2023)