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Literature summary for 1.4.1.14 extracted from

  • Hayakawa, T.; Yamaya, T.; Kamachi, K.; Ojima, K.
    Purification, characterization, and immunological properties of NADH-dependent glutamate synthase from rice cell cultures (1992), Plant Physiol., 98, 1317-1322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.14

Inhibitors

Inhibitors Comment Organism Structure
azaserine completely inhibited by 4 mM Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 NADH
-
 Oryza sativa
0.076
-
 2-oxoglutarate
-
 Oryza sativa
0.811
-
 L-glutamine
-
 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
194000
-
 gel filtration Oryza sativa
196000
-
 1 * 196000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Oryza sativa role in synthesis of glutamate for growth and development in higher plants glutamate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
815fold purification Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.8
-
-
 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Oryza sativa L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH specific for NADH Oryza sativa L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH role in synthesis of glutamate for growth and development in higher plants Oryza sativa glutamate + NAD+
-
 ?
additional information no activity with L-asparagine, NH4Cl or NADPH Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 196000, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
 L-glutamine
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
flavin flavoprotein Oryza sativa
additional information no activity with NADPH Oryza sativa
NADH specific for NADH as reductant Oryza sativa