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Literature summary for 1.4.1.14 extracted from
- Some properties of glutamine synthetase and glutamate synthase from Derxia gummosa (1985), Phytochemistry, 24, 1133-1139.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 10 mM, 84% inhibition | Derxia gummosa |  |
| AMP | 10 mM, 61% inhibition | Derxia gummosa | |
| aspartate | 10 mM, 60% inhibition | Derxia gummosa | |
| ATP | 10 mM, 37% inhibition | Derxia gummosa | |
| azaserine | - |
Derxia gummosa | |
| L-glutamate | 10 mM, 24% inhibition | Derxia gummosa | |
| methionine | 10 mM, 47% inhibition | Derxia gummosa | |
| additional information | not inhibited by alanine, glycine, serine, lysine, proline, cysteine, valine, leucine, isoleucine, threonine, phenylalanine, histidine, tryptophan, asparagine, arginine, UTP, ITP, GTP | Derxia gummosa | |
| NADPH | 0.2 mM, 50% inhibition | Derxia gummosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0096 | - |
NADH | - |
Derxia gummosa | |
| 0.024 | - |
2-oxoglutarate | - |
Derxia gummosa | |
| 0.27 | - |
L-glutamine | - |
Derxia gummosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Derxia gummosa | - |
obligate aerobic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 167fold purification | Derxia gummosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Derxia gummosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Derxia gummosa | L-glutamate + NAD+ | - |
? | |
| L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Derxia gummosa | L-glutamate + NAD+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Derxia gummosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.2 | optimal pH-range with maximum at pH 7.5 | Derxia gummosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | specific for NADH as reductant | Derxia gummosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
azaserine | - |
Derxia gummosa | |
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