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Literature summary for 1.4.1.14 extracted from

  • Boland, M.J.; Court, C.B.
    Glutamate synthase (NADH) from lupin nodules. Specificity of the 2-oxoglutarate site (1981), Biochim. Biophys. Acta, 657, 539-542.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyglutarate D- and L-isomer, most potent competitive inhibitor Lupinus angustifolius
2-oxoglutarate
-
Lupinus angustifolius
aspartate weak inhibition, competitive with 2-oxoglutarate Lupinus angustifolius
D-2-hydroxyglutarate
-
Lupinus angustifolius
D-glutamate competitive inhibitor Lupinus angustifolius
Glutarate most potent competitive inhibitor Lupinus angustifolius
L-2-hydroxyglutarate
-
Lupinus angustifolius
L-glutamate competitive with 2-oxoglutarate Lupinus angustifolius
oxaloacetate weak inhibition, competitive with 2-oxoglutarate Lupinus angustifolius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
2-oxoglutarate
-
Lupinus angustifolius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Lupinus angustifolius ammonia assimilation from fixed nitrogen, de novo synthesis of glutamate glutamate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Lupinus angustifolius
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
Lupinus angustifolius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH absolute specificity for 2-oxoglutarate Lupinus angustifolius L-glutamate + NAD+
-
?
L-glutamine + 2-oxoglutarate + NADH ammonia assimilation from fixed nitrogen, de novo synthesis of glutamate Lupinus angustifolius glutamate + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Lupinus angustifolius

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
L-2-hydroxyglutarate
-
Lupinus angustifolius
0.28
-
D-2-hydroxyglutarate
-
Lupinus angustifolius
0.35
-
Glutarate
-
Lupinus angustifolius
0.6
-
L-glutamate
-
Lupinus angustifolius
0.6
-
D-glutamate
-
Lupinus angustifolius
2.7
-
L-aspartate
-
Lupinus angustifolius
5
-
oxaloacetate
-
Lupinus angustifolius