Literature summary for 1.4.1.16 extracted from

Misono, H.; Soda, K.
Properties of meso-alpha,epsilon-diaminopimelate D-dehydrogenase from Bacillus sphaericus (1980), J. Biol. Chem., 255, 10599-10605.

Search for more literature for 1.4.1.16

Inhibitors

Inhibitors	Comment	Organism	Structure
D-2,6-diaminopimelate	-	Lysinibacillus sphaericus
glyoxylate	5 mM, 30% inhibition of reductive amination	Lysinibacillus sphaericus
HgCl2	0.01 mM, complete inhibition	Lysinibacillus sphaericus
L-2,6-diaminopimelate	-	Lysinibacillus sphaericus
L-alpha,epsilon-diaminopimelate	-	Lysinibacillus sphaericus
meso-alpha,delta-Diaminoadipate	-	Lysinibacillus sphaericus
p-chloromercuribenzoate	0.001 mM, complete inhibition	Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00083	-	NADP+	-	Lysinibacillus sphaericus
0.083	0.14	NADP+	-	Lysinibacillus sphaericus
0.2	-	NADPH	-	Lysinibacillus sphaericus
0.24	-	L-2-amino-6-oxopimelate	-	Lysinibacillus sphaericus
2.5	-	meso-2,6-diaminopimelate	-	Lysinibacillus sphaericus
12.5	-	NH3	-	Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	2 * 41000, SDS-PAGE	Lysinibacillus sphaericus
78500	80000	gel filtration	Lysinibacillus sphaericus
78500	80000	sedimentation equilibrium centrifugation	Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NADPH	Lysinibacillus sphaericus	-	meso-2,6-diaminoheptanedioate + H2O + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150	Lysinibacillus sphaericus

Reaction

Reaction	Comment	Organism	Reaction ID
meso-2,6-diaminoheptanedioate + H2O + NADP+ = L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+	catalyzes the transfer of the pro-S hydrogen at C-4 of NADPH to the substrate, enzyme is B-stereospecific	Lysinibacillus sphaericus	a

Storage Stability

Storage Stability	Organism
-20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year	Lysinibacillus sphaericus
4°C, 0.01% 2-mercaptoethanol, 20% glycerol, 1 month	Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H	-	Lysinibacillus sphaericus	meso-2,6-diaminoheptanedioate + H2O + NAD(P)+	-	r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH	-	Lysinibacillus sphaericus	meso-2,6-diaminoheptanedioate + H2O + NADP+	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 41000, SDS-PAGE	Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	unstable above	Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	2-amino-6-oxopimelate + NH3 + NADPH	Lysinibacillus sphaericus
10.5	-	meso-2,6-diaminopimelate + H2O + NADP+	Lysinibacillus sphaericus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.9	10	2-amino-6-oxopimelate + NH3 + NADPH	Lysinibacillus sphaericus
6.8	11	meso-2,6-diaminopimelate + H2O + NADP+	Lysinibacillus sphaericus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	-	Lysinibacillus sphaericus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Lysinibacillus sphaericus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.12	-	L-alpha,epsilon-diaminopimelate	-	Lysinibacillus sphaericus
4	-	D-alpha,epsilon-diaminopimelate	-	Lysinibacillus sphaericus
4.16	-	meso-alpha,delta-Diaminoadipate	-	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)