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Literature summary for 1.4.1.16 extracted from

  • Cheng, X.; Chen, X.; Feng, J.; Wu, Q.; Zhu, D.
    Structure-guided engineering of meso-diaminopimelate dehydrogenase for enantioselective reductive amination of sterically bulky 2-keto acids (2018), Catal. Sci. Technol., 8, 4994-5002 .
No PubMed abstract available
Search for more literature for 1.4.1.16

Protein Variants

Protein Variants Comment Organism
H227I mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
H227V mutant shows high activity toward phenylpyruvic acid and 2-oxo-4-phenylbutyric acid Symbiobacterium thermophilum
T171H mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
W121L mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
W121L/H227I mutant sow improved enzyme activities towards various 2-oxoacids including sterically bulky substrates. The substrate binding cavity of the mutant enzyme is reshaped to accommodate these bulky substrates, thus leading to higher enzyme activity Symbiobacterium thermophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.2
-
 2-oxo-3-phenylpropanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
17.72
-
 2-oxo-4-methylpentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
23.5
-
 2-oxopentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
33.83
-
 2-oxo-4-phenylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
35.12
-
 2-oxobutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
46.1
-
 2-oxo-3-methylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
48.46
-
 pyruvate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum Q67PI3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo(phenyl)acetate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-amino(phenyl)acetate + NADP+
-
 r
2-oxo-3,3-dimethylbutanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-3,3-dimethylbutanoate + NADP+
-
 r
2-oxo-3-methylbutanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-3-methylbutanoate + NADP+
-
 r
2-oxo-3-phenylpropanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-3-phenylpropanoate + NADP+
-
 r
2-oxo-4-methylpentanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-4-methylpentanoate + NADP+
-
 r
2-oxo-4-phenylbutanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-4-phenylbutanoate + NADP+
-
 r
2-oxobutanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-aminobutanoate + NADP+
-
 r
2-oxopentanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-aminopentanoate + NADP+
-
 r
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum (2S)-2-amino-3-(1H-indol-3-yl)propanoate + NADP+
-
 r
pyruvate + NH3 + NADPH + H+
-
 Symbiobacterium thermophilum D-alanine + NADP+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.17
-
 2-oxo-3-methylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
6.2
-
 2-oxo-3-phenylpropanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
12.6
-
 2-oxo-4-phenylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
13.49
-
 2-oxo-4-methylpentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
35.38
-
 2-oxobutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
38.7
-
 2-oxopentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
45.22
-
 pyruvate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum