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Literature summary for 1.4.1.2 extracted from

  • Scheid, H.W.; Ehmke, A.; Hartmann, T.
    Plant NAD-dependent glutamate dehydrogenase. Purification, molecular properties and metal ion activation of the enzymes from Lemna minor and Pisum sativum (1980), Z. Naturforsch. C, 35, 213-221.
    View publication on PubMed
Search for more literature for 1.4.1.2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pisum sativum
Ca2+ required Lemna minor
Zn2+ can replace Ca2+, requirement Pisum sativum
Zn2+ can replace Ca2+, requirement Lemna minor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58500
-
 4 * 58500, SDS-PAGE Pisum sativum
58500
-
 4 * 58500, SDS-PAGE Lemna minor
230000
-
 gel filtration Lemna minor
230000
-
 sedimentation equilibrium analysis Pisum sativum

Organism

Organism UniProt Comment Textmining
Lemna minor
-
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum
-
 Lemna minor

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
401
-
-
 Pisum sativum
540
-
-
 Lemna minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Pisum sativum 2-oxoglutarate + NH3 + NADH + H+
-
 ?
L-glutamate + H2O + NAD+
-
 Lemna minor 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58500, SDS-PAGE Pisum sativum
tetramer 4 * 58500, SDS-PAGE Lemna minor

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pisum sativum
NAD+
-
 Lemna minor
NADH
-
 Pisum sativum
NADH
-
 Lemna minor
NADP+ less than 1% of the activity with NAD+ Lemna minor
NADPH
-
 Pisum sativum
NADPH
-
 Lemna minor