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Literature summary for 1.4.1.2 extracted from

  • Camardella, L.; Di Fraia, R.; Antignani, A.; Ciardiello, M.A.; di Prisco, G.; Coleman, J.K.; Buchon, L.; Guespin, J.; Russell, N.J.
    The Antarctic Psychrobacter sp. TAD1 has two cold-active glutamate dehydrogenases with different cofactor specificities. Characterisation of the NAD+-dependent enzyme (2002), Comp. Biochem. Physiol. A, 131, 559-567.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 NAD+ NAD+-dependent enzyme Psychrobacter sp.
2.36
-
 2-oxoglutarate NAD+-dependent enzyme Psychrobacter sp.
24.6
-
 NH4+ NAD+-dependent enzyme Psychrobacter sp.
28.6
-
 L-glutamate NAD+-dependent enzyme Psychrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 NAD+-dependent enzyme, 2 * 160000, SDS-PAGE Psychrobacter sp.
300000
-
 NAD+-dependent enzyme, gel filtration Psychrobacter sp.

Organism

Organism UniProt Comment Textmining
Psychrobacter sp.
-
 TAD 1, two enzymes
-

Purification (Commentary)

Purification (Comment) Organism
NAD+-dependent and NADP+-dependent GDH Psychrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
248
-
 NAD+-dependent enzyme Psychrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
 Psychrobacter sp. L-glutamate + NADP+
-
 ?
L-glutamate + H2O + NAD+
-
 Psychrobacter sp. 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer NAD+-dependent enzyme, 2 * 160000, SDS-PAGE Psychrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 NAD+-dependent enzyme Psychrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Psychrobacter sp.
NADP+
-
 Psychrobacter sp.