KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
NAD+ | pH 8.0, 20°C | Psychrobacter sp. | |
2.36 | - |
2-oxoglutarate | pH 8.0, 20°C | Psychrobacter sp. | |
24.6 | - |
NH3 | pH 8.0, 20°C | Psychrobacter sp. | |
28.6 | - |
L-glutamate | pH 8.0, 20°C | Psychrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
2 * 160000, SDS-PAGE | Psychrobacter sp. |
300000 | - |
gel filtration | Psychrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter sp. | - |
- |
- |
Psychrobacter sp. TAD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Psychrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
248 | - |
- |
Psychrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Psychrobacter sp. | L-glutamate + NAD+ + H2O | - |
r | |
2-oxoglutarate + NADH + NH3 | - |
Psychrobacter sp. TAD1 | L-glutamate + NAD+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 160000, SDS-PAGE | Psychrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent GDH | - |
Psychrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Psychrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 43 | 10°C: about 45% of maximal activity, 43°C: about 75% of maximal activity | Psychrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
about 45% loss of activity after 15 min, about 60% loss of activity after 30 min | Psychrobacter sp. |
40 | - |
about 60% loss of activity after 5 min, about 80% loss of activity after 15 min | Psychrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Psychrobacter sp. | |
NADH | - |
Psychrobacter sp. |