Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4NO3 | increases the NADH-GDH activity in the presence of Hg | Phaseolus vulgaris |
Application | Comment | Organism |
---|---|---|
additional information | possible role of enzyme under Hg-stress | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | inhibits the enzyme activity in the absence of Hg | Phaseolus vulgaris | |
glutamine | reduces increase in GDH activity due to Hg | Phaseolus vulgaris | |
glutathione | reduces increase in GDH activity due to Hg | Phaseolus vulgaris | |
additional information | increase in GDH activity due to Hg remains unaffected by the supply of sucrose | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | enhances increase in GDH activity due to Hg | Phaseolus vulgaris | |
Hg2+ | increases NADH-GDH activity substantially, however, specific activity of the enzyme decreases at lower concentration of Hg, and increases to lesser extent at higher concentration of Hg | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.7 | - |
in the absence of Hg and in the presence of 0.1 mM AlCl3 | Phaseolus vulgaris |
25 | - |
in the presence of 0.001 mM Hg and in the presence of NH4NO3 | Phaseolus vulgaris |
26.7 | - |
in the presence of 0.001 mM Hg | Phaseolus vulgaris |
34.2 | - |
in the absence of Hg | Phaseolus vulgaris |
52.9 | - |
in the absence of Hg and in the presence of 5 mM sucrose | Phaseolus vulgaris |
54.6 | - |
in the presence of 0.01 mM Hg | Phaseolus vulgaris |
58.6 | - |
in the absence of Hg and in the presence of 5 mM glutathione | Phaseolus vulgaris |
59.4 | - |
in the absence of Hg and in the presence of 5 mM glutamine | Phaseolus vulgaris |
65.8 | - |
in the absence of Hg and in the presence of NH4NO3 | Phaseolus vulgaris |
70.94 | - |
in the presence of 0.1 mM Hg and in the presence of 5 mM glutathione | Phaseolus vulgaris |
72 | - |
in the presence of 0.01 mM Hg and in the presence of NH4NO3 | Phaseolus vulgaris |
119.5 | - |
in the presence of 0.1 mM Hg | Phaseolus vulgaris |
170.6 | - |
in the presence of 0.1 mM Hg and in the presence of 5 mM glutamine | Phaseolus vulgaris |
171.4 | - |
in the presence of 0.1 mM Hg and in the presence of 0.1 mM AlCl3 | Phaseolus vulgaris |
243.4 | - |
in the presence of 0.1 mM Hg and in the presence of NH4NO3 | Phaseolus vulgaris |
328.2 | - |
in the presence of 0.1 mM Hg and in the presence of 5 mM sucrose | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Phaseolus vulgaris | L-glutamate + H2O + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADH-GDH | - |
Phaseolus vulgaris |
NADH-glutamate dehydrogenase | - |
Phaseolus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | gradual increase in enzyme activity with increasing concentrations of NADH in preparations with 0.1 mM Hg or without Hg | Phaseolus vulgaris |