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Literature summary for 1.4.1.2 extracted from

  • Watanabe, M.; Yumi, O.; Itoh, Y.; Yasuda, K.; Kamachi, K.; Ratcliffe, R.G.
    Deamination role of inducible glutamate dehydrogenase isoenzyme 7 in Brassica napus leaf protoplasts (2011), Phytochemistry, 72, 587-593.
    View publication on PubMed
Search for more literature for 1.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
jasmonic acid
-
 Brassica napus
salicylic acid
-
 Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Brassica napus 5739
-
protoplast isozyme GDH7 predominates Brassica napus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Brassica napus
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 GDH isoenzymes 1 and 7, genes GDH1 and GDH2 encoding subunits alpha and beta
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf the isoenzyme profile in leaves changes on wounding Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Brassica napus 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
hexamer (alphabeta)3 Brassica napus

Synonyms

Synonyms Comment Organism
NAD+-dependent GDH
-
 Brassica napus
NAD+-dependent glutamate dehydrogenase
-
 Brassica napus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Brassica napus
NADH
-
 Brassica napus

Expression

Organism Comment Expression
Brassica napus transcription of GDH2, which encodes the GDH alpha subunit, is activated and translation of the GDH2 mRNA is also activated to synthesize a subunit polypeptides, when protoplasts are isolated. When detached leaves absorb either acidic 5 mM jasmonic acid or salicylic acid solutions via petioles, GDH7 isoenzyme is activated and the GDH isoenzyme expression pattern is similar to that of protoplasts up

General Information

General Information Comment Organism
additional information the isoenzyme profile in leaves changes on wounding, the change in GDH isoenzyme profile has no effect on ammonium assimilation. Protoplast isolation changes the redox state with NAD(P)H and oxidized glutathione levels increasing, and ascorbate, dehydroascorbate, NAD(P)+ and glutathione decreasing. ATP content in protoplasts declines to 2.6% of that in leaves, while that in wounded leaves increases by twofold Brassica napus
physiological function isozyme GDH7 does not support net amination in vivo and the increase in GDH7 activity might be a response to oxidative stress during protoplast isolation Brassica napus