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Literature summary for 1.4.1.2 extracted from

  • Mara, P.; Fragiadakis, G.S.; Gkountromichos, F.; Alexandraki, D.
    The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae (2018), Microb. Cell Fact., 17, 170 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
gene gdh2, GDH2 is encoded on chromosome IV Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Saccharomyces cerevisiae
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+ Saccharomyces cerevisiae ATCC 204508
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P33327
-
-
Saccharomyces cerevisiae ATCC 204508 P33327
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein isozyme Gdh2p can be converted from an active NAD-dependent glutamate dehydrogenase to an inactive form by phosphorylation through cAMP-dependent and cAMP-independent protein kinases Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Saccharomyces cerevisiae 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+
-
 Saccharomyces cerevisiae ATCC 204508 2-oxoglutarate + NH3 + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
GDH2
-
 Saccharomyces cerevisiae
Gdh2p
-
 Saccharomyces cerevisiae
glutamate dehydrogenase 2
-
 Saccharomyces cerevisiae
NAD-dependent glutamate dehydrogenase
-
 Saccharomyces cerevisiae
NAD-GDH
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharomyces cerevisiae
NADH
-
 Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae in the presence of preferred nitrogen sources, the expression of GDH2 is repressed by the transcriptional regulator Ure2 which sequesters Gln3 into the cytoplasm. The expression of GDH2 is regulated by the concurrent action of Gcn4 and Gln3. These two regulatory networks have been thought to interact, putting forward the existence of a physiological relation between Gln3 and Gcn4. Under nitrogen derepressive conditions and amino acid deprivation, Gcn4 and Gln3 form part of a transcriptional complex that binds on GDH2 promoter and dictates its expression. Overexpression of GDH2 is favoring yeast growth providing a growth advantage down

General Information

General Information Comment Organism
malfunction the disruption of GDH2 was not deleterious to glutamate homeostasis. Mutant gdh2DELTA cells present wild-type growth and do not display any deficiencies due to glutamate homeostasis impairment neither under glucose nor under non-fermentable carbon sources. Deletion of GDH2 gene in a gdh3DELTA background increases the resistance under thermal or oxidative stress by decreasing ROS accumulation. The apoptosis is suppressed by GDH2 deletion through the elevated levels of glutamate and glutathione present in the double mutant. Under the tested conditions, deletion of GDH2 compensates the depletion of intracellular glutamate and glutathione (GSH) followed by stress-induced apoptotic cell death reinforcing further the idea that Gdh2p is responsible only for glutamate catabolism and not its production Saccharomyces cerevisiae
metabolism through the enzymatic activity of Gdh2p the breakdown of glutamate provides adequate levels of ammonia in yeast cells. The catabolism of glutamate via the NAD-GDH activity is the major pathway of ammonia generation in vivo. Synthesis of glutamate occurs through the action of NADP-GDH (encoded by GDH1 and GDH3 genes, EC 1.4.1.4). NAD-GDH activity (encoded by GDH2) is responsible for glutamate degradation and release of ammonium and 2-oxoglutarate. The role of GDH1 and GDH2 is contradictory when investigated in yeast strains under cold-growth conditions Saccharomyces cerevisiae
physiological function the NAD-GDH activity in yeast is encoded by GDH2 gene and catalyzes the oxidative deamination of glutamate to 2-oxoglutarate and ammonium. Yeast cells lacking GDH1 can use GDH2 to promote glutamate biosynthesis using ammonia as sole nitrogen source. Role of the GDH path in ROS-mediated apoptosis. GDH2 genetically interacts with GDH3 (EC 1.4.1.4) and controls stress-induced apoptosis. Role of GDH2 in glutamate homeostasis. GDH2 genetically interacts with GDH3 and controls stress-induced apoptosis Saccharomyces cerevisiae