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Literature summary for 1.4.1.20 extracted from

  • Hummel, W.; Schuette, H.; Schmidt, E.; Wandrey, C.; Kula, M.R.
    Isolation of L-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of L-phenylalanine (1987), Appl. Microbiol. Biotechnol., 26, 409-416.
No PubMed abstract available
Search for more literature for 1.4.1.20

Application

Application Comment Organism
synthesis continous production of L-Phe in an enzyme-membrane-reactor Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
L-Phe
-
 Rhodococcus sp.
NAD+
-
 Rhodococcus sp.
NADH
-
 Rhodococcus sp.
phenylpyruvate
-
 Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 NADH
-
 Rhodococcus sp.
0.16
-
 phenylpyruvate
-
 Rhodococcus sp.
0.27
-
 NAD+
-
 Rhodococcus sp.
0.43
-
 L-Met
-
 Rhodococcus sp.
0.75
-
 L-Phe
-
 Rhodococcus sp.
2.1
-
 2-oxo-4-methylthiobutanoate
-
 Rhodococcus sp.
2.4
-
 p-hydroxyphenylpyruvate
-
 Rhodococcus sp.
3.1
-
 L-Tyr
-
 Rhodococcus sp.
7.7
-
 Indolepyruvate
-
 Rhodococcus sp.
11
-
 L-Trp
-
 Rhodococcus sp.
387
-
 NH4+
-
 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 M4
-
Rhodococcus sp. M4
-
 M4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-methylthiobutanoate + NH3 + NADH 33% of the activity with L-Phe Rhodococcus sp. L-Met + NAD+ + H2O
-
 ?
4-hydroxyphenylpyruvate + NH3 + NADH 5% of the activity with L-Phe Rhodococcus sp. L-Tyr + NAD+ + H2O
-
 ?
4-hydroxyphenylpyruvate + NH3 + NADH 5% of the activity with L-Phe Rhodococcus sp. M4 L-Tyr + NAD+ + H2O
-
 ?
indolepyruvate + NH3 + NADH 3% of the activity with L-Phe Rhodococcus sp. ?
-
 ?
L-Met + H2O + NAD+ 4% of the activity with L-Phe Rhodococcus sp. 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
 ?
L-Met + H2O + NAD+ 4% of the activity with L-Phe Rhodococcus sp. M4 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
 ?
L-Phe + H2O + NAD+
-
 Rhodococcus sp. phenylpyruvate + NH3 + NADH
-
 r
L-Phe + H2O + NAD+
-
 Rhodococcus sp. M4 phenylpyruvate + NH3 + NADH
-
 r
L-Trp + H2O + NAD+ 2% of the activity with L-Phe Rhodococcus sp. indole-3-pyruvate + NH3 + NADH
-
 ?
L-Trp + H2O + NAD+ 2% of the activity with L-Phe Rhodococcus sp. M4 indole-3-pyruvate + NH3 + NADH
-
 ?
L-Tyr + H2O + NAD+
-
 Rhodococcus sp. 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
L-Tyr + H2O + NAD+
-
 Rhodococcus sp. M4 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 50% of maximal activity, 70°C: about 70% of maximal activity Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
 reductive amination Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhodococcus sp.
NADH
-
 Rhodococcus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 NADH
-
 Rhodococcus sp.
0.07
-
 phenylpyruvate
-
 Rhodococcus sp.
1.27
-
 NAD+
-
 Rhodococcus sp.
7.34
-
 phenylpyruvate
-
 Rhodococcus sp.
17.85
-
 Phe
-
 Rhodococcus sp.