Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NAD+ | - |
Lysinibacillus sphaericus | |
0.24 | - |
L-phenylalanine | - |
Lysinibacillus sphaericus | |
0.48 | - |
L-tyrosine | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
8 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
340000 | - |
gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Lysinibacillus sphaericus | - |
phenylpyruvate + NH3 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PheDH, ammonium sulfate, DEAE-Toyopearl, hydroxyapatite, Sephadex G-200 | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
285.4 | - |
recombinant PheDH, oxidative deamination | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + NAD+ | 1% of activity with L-phenylalanine | Lysinibacillus sphaericus | 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH | - |
r | |
L-methionine + H2O + NAD+ | 3% of activity with L-phenylalanine | Lysinibacillus sphaericus | (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH | - |
r | |
L-norleucine + H2O + NAD+ | 4.5% of activity with L-phenylalanine | Lysinibacillus sphaericus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | phenylpyruvate + NH3 + NADH | - |
r | |
L-tryptophan + H2O + NAD+ | 2% of activity with L-phenylalanine | Lysinibacillus sphaericus | 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADH | - |
r | |
L-tyrosine + H2O + NAD+ | 74% of activity with L-phenylalanine | Lysinibacillus sphaericus | (4-hydroxyphenyl)pyruvate + NH3 + NADH | - |
r | |
L-valine + H2O + NAD+ | 1.8% of activity with L-phenylalanine | Lysinibacillus sphaericus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.2 | - |
reductive amination | Lysinibacillus sphaericus |
11 | - |
oxidative deamination | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Lysinibacillus sphaericus |