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Literature summary for 1.4.1.20 extracted from

  • Omidinia, E.; Samadi, A.; Taherkhani, H.; Khatami, S.; Moazami, N.; Pouraie, R.R.; Asano, Y.
    Cloning and expression of Bacillus sphaericus phenylalanine dehydrogenase gene in Bacillus subtilis cells: purification and enzyme properties (2002), World J. Microbiol. Biotechnol., 18, 593-597.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
NAD+
-
Lysinibacillus sphaericus
0.24
-
L-phenylalanine
-
Lysinibacillus sphaericus
0.48
-
L-tyrosine
-
Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
8 * 41000, SDS-PAGE Lysinibacillus sphaericus
340000
-
gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + H2O + NAD+ Lysinibacillus sphaericus
-
phenylpyruvate + NH3 + NADH
-
r

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PheDH, ammonium sulfate, DEAE-Toyopearl, hydroxyapatite, Sephadex G-200 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
285.4
-
recombinant PheDH, oxidative deamination Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + H2O + NAD+ 1% of activity with L-phenylalanine Lysinibacillus sphaericus 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH
-
r
L-methionine + H2O + NAD+ 3% of activity with L-phenylalanine Lysinibacillus sphaericus (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
r
L-norleucine + H2O + NAD+ 4.5% of activity with L-phenylalanine Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
r
L-phenylalanine + H2O + NAD+
-
Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
r
L-tryptophan + H2O + NAD+ 2% of activity with L-phenylalanine Lysinibacillus sphaericus 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADH
-
r
L-tyrosine + H2O + NAD+ 74% of activity with L-phenylalanine Lysinibacillus sphaericus (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
r
L-valine + H2O + NAD+ 1.8% of activity with L-phenylalanine Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 41000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
PheDH
-
Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.2
-
reductive amination Lysinibacillus sphaericus
11
-
oxidative deamination Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Lysinibacillus sphaericus