Literature summary for 1.4.1.20 extracted from

Cainelli, G.; Engel, P.C.; Galletti, P.; Giacomini, D.; Gualandi, A.; Paradisi, F.
Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions (2005), Org. Biomol. Chem., 3, 4316-4320.

Search for more literature for 1.4.1.20

Application

Application	Comment	Organism
biotechnology	application of the immobilised mutant enzyme N145A that is remarkably robust, even in the presence of high concentrations of polar or non-polar organic solvents such as acetone, methanol, n-hexane, toluene and methylene chloride in the synthesis of p-NO2-phenylalanine from the poorly water-soluble p-NO2-phenylpyruvic acid. 100% stereoselectivity	Lysinibacillus sphaericus

Protein Variants

Protein Variants	Comment	Organism
N145A	the immobilised biocatalyst is remarkably robust, even in the presence of high concentrations of polar or non-polar organic solvents such as acetone, methanol, n-hexane, toluene and methylene chloride	Lysinibacillus sphaericus

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitro-phenylpyruvate + NH3 + NADH	reaction with the immobilized mutant enzyme N145A	Lysinibacillus sphaericus	4-nitro-L-phenylalanine + H2O + NAD+	-	?

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Release 2023.1 (January 2023)