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Literature summary for 1.4.1.20 extracted from

  • Jiang, W.; Sun, D.; Ren, H.; Xu, C.; Wang, Y.; Zhang, Y.; Wang, S.; Fang, B.
    Isolation, purification and characterization of a salt-active and organic-solvent-thermostable phenylalanine dehydrogenase from Bacillus nanhaiensis DSF-15A2 (2016), J. Mol. Catal. B, 133, 12-19 .
No PubMed abstract available
Search for more literature for 1.4.1.20

Activating Compound

Activating Compound Comment Organism Structure
ethylene glycolmonomethyl ether the catalytic efficiency in ethylene glycolmonomethyl ether is 1.15- and 1.35fold than that of in the aqueous phase Fictibacillus nanhaiensis
methanol the catalytic efficiency in 30% (v/v) methanol is 1.15fold than that of in the aqueous phase Fictibacillus nanhaiensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Fictibacillus nanhaiensis

General Stability

General Stability Organism
after pre-treatment with 1 M NaCl at 55°C for 1 h, the enzyme exhibits high activity and retains over 50% of its maximal activity during pH 7.0-8.5 Fictibacillus nanhaiensis

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile in the presence of acetonitrile at 10% (v/v), the enzyme retains about 60% of the original activity. However, the enzyme exhibits nearly no activity in the presence of acetonitrile at 30% (v/v) Fictibacillus nanhaiensis
Isopropanol in the presence of isopropanol at 10% (v/v), the enzyme retains about 30% of the original activity. However, the enzyme exhibits nearly no activity in the presence of isopropanol at 30% (v/v) Fictibacillus nanhaiensis
additional information the enzyme exhibits nearly no change on enzyme activity in the presence of glycol, ethanol and methanol at 10% (v/v) Fictibacillus nanhaiensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information salt ions have little effect on enzyme activity Fictibacillus nanhaiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + H2O + NAD+ Fictibacillus nanhaiensis
-
 phenylpyruvate + NH3 + NADH + H+
-
 ?
L-phenylalanine + H2O + NAD+ Fictibacillus nanhaiensis DSF-15A2
-
 phenylpyruvate + NH3 + NADH + H+
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the enzyme shows high activity in the presence of acetone from 10% (v/v) to 30% (v/v), retaining 100% of the original activity Fictibacillus nanhaiensis
ethylene glycol monomethyl ether the enzyme shows high activity in the presence of ethylene glycol monomethyl ether from 10% (v/v) to 30% (v/v), retaining 100% of the original activity Fictibacillus nanhaiensis

Organism

Organism UniProt Comment Textmining
Fictibacillus nanhaiensis A0A1B1PGG4
-
-
Fictibacillus nanhaiensis DSF-15A2 A0A1B1PGG4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA column chromatography Fictibacillus nanhaiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O + NAD+
-
 Fictibacillus nanhaiensis phenylpyruvate + NH3 + NADH + H+
-
 ?
L-phenylalanine + H2O + NAD+
-
 Fictibacillus nanhaiensis DSF-15A2 phenylpyruvate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x 40710, His-tagged enzyme, calculated from amino acid sequence Fictibacillus nanhaiensis

Synonyms

Synonyms Comment Organism
PheDH
-
 Fictibacillus nanhaiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Fictibacillus nanhaiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 60 at temperatures from 0 to 60°C, the enzyme retains over 70% of its maximal activity at 40°C Fictibacillus nanhaiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 55 after incubation at 30-55°C for 1 h under pH 10.0, the enzyme retains over 60% of its maximal activity while it retains about no activity at 55°C Fictibacillus nanhaiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Fictibacillus nanhaiensis

pH Range

pH Minimum pH Maximum Comment Organism
9 10.5 the enzyme shows more than 50% of the maximal activity between pH 9.0 and 10.5 Fictibacillus nanhaiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10.5 without any stabilizer, all samples except at pH 9.5, 10.0 and 6.0 maintain over 100% activity after being pre-treated in buffers ranging from pH 7.0-10.5 for 1 h at 30°C. The enzyme keeps more than 100% of its maximal activity after 1 h at 40°C (pH 7.0-9.0). Furthermore, it is stable under weak alkaline conditions (pH 7.0-9.0) after being pre-treated for 1 h at 50°C Fictibacillus nanhaiensis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Fictibacillus nanhaiensis