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Literature summary for 1.4.1.20 extracted from

  • Jiang, W.; Wang, Y.L.; Fang, B.S.
    Resolution mechanism and characterization of an ammonium chloride-tolerant, high-thermostable, and salt-tolerant phenylalanine dehydrogenase from Bacillus halodurans (2018), Appl. Biochem. Biotechnol., 186, 789-804 .
    View publication on PubMed
Search for more literature for 1.4.1.20

Activating Compound

Activating Compound Comment Organism Structure
NaCl 2 h in 3 M NaCl at 60 °C, the residual activity is 1.8fold higher than that of the control without NaCl Halalkalibacterium halodurans
NH4+ enzyme tolerates high concentrations of ammonium chloride and its activity can also be enhanced by high concentration of ammonium salts Halalkalibacterium halodurans

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans Q9KG94
-
-
Halalkalibacterium halodurans DSM 18197 Q9KG94
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
 oxidative deamination, substrate phnylpyruvate, pH 10.4, 25°C Halalkalibacterium halodurans
26.6
-
 reductive amination, substrate phenylpyruvate, pH 10.4, 25°C Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O + NAD+
-
 Halalkalibacterium halodurans phenylpyruvate + NH3 + NADH + H+
-
 ?
L-phenylalanine + H2O + NAD+
-
 Halalkalibacterium halodurans DSM 18197 phenylpyruvate + NH3 + NADH + H+
-
 ?
phenylpyruvate + NH3 + NADH + H+
-
 Halalkalibacterium halodurans L-phenylalanine + H2O + NAD+
-
 ?
phenylpyruvate + NH3 + NADH + H+
-
 Halalkalibacterium halodurans DSM 18197 L-phenylalanine + H2O + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 41800, calculated from sequence Halalkalibacterium halodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 reductive amination Halalkalibacterium halodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 2 h incubation, pH 7.0, enzyme retaines approximately 80% of maximum activity Halalkalibacterium halodurans
70
-
 2 h, less than 30% residual activity Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Halalkalibacterium halodurans

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 more than 70% of maximum activity Halalkalibacterium halodurans