Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Fervidobacterium nodosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R, 4S)-2,4-diaminopentanoate | uncompetitive substrate inhibition | Fervidobacterium nodosum | |
Ca2+ | 85% residual activity at 0.5 mM | Fervidobacterium nodosum | |
Co2+ | 34% residual activity at 0.5 mM | Fervidobacterium nodosum | |
Cu2+ | complete inhibition at 0.5 mM | Fervidobacterium nodosum | |
D-alanine | enzymatic activity decreases to 71% of the positive control at 5 mM | Fervidobacterium nodosum | |
D-Lysine | enzymatic activity decreases to 68% of the positive control at 5 mM | Fervidobacterium nodosum | |
D-ornithine | uncompetitive inhibition, enzymatic activity decreases to 14% of the positive control at 5 mM | Fervidobacterium nodosum | |
Fe2+ | 84% residual activity at 0.5 mM | Fervidobacterium nodosum | |
Mg2+ | 65% residual activity at 0.5 mM | Fervidobacterium nodosum | |
additional information | EDTA does not affect the enzyme activity | Fervidobacterium nodosum | |
Ni2+ | 2% residual activity at 0.5 mM | Fervidobacterium nodosum | |
Zn2+ | 2% residual activity at 0.5 mM | Fervidobacterium nodosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | at pH 8.5 and 55°C | Fervidobacterium nodosum | |
0.2 | - |
(2R,4S)-2,4-diaminopentanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum | |
2 | - |
NADP+ | at pH 8.5 and 55°C | Fervidobacterium nodosum | |
2 | - |
2,5-Diaminohexanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | K+ and Mn2+ have no significant effect on the enzyme activity | Fervidobacterium nodosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+ | Fervidobacterium nodosum | - |
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | - |
? | |
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+ | Fervidobacterium nodosum | - |
(2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fervidobacterium nodosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Bind column chromatography | Fervidobacterium nodosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
with 2,5-diaminohexanoate as substrate, at pH 8.5 and 55°C | Fervidobacterium nodosum |
92 | - |
with (2R,4S)-2,4-diaminopentanoate as substrate, at pH 8.5 and 55°C | Fervidobacterium nodosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+ | - |
Fervidobacterium nodosum | (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | - |
? | |
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+ | - |
Fervidobacterium nodosum | (2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+ | - |
? | |
2,5-diaminohexanoate + H2O + NAD+ | 90fold less activity with 2,5-diaminohexanoate compared to (2R,4S)-2,4-diaminopentanoate | Fervidobacterium nodosum | 2-amino-5-oxohexanoate + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Fervidobacterium nodosum |
homodimer | 2 * 38000, calculated from amino acid sequence | Fervidobacterium nodosum |
Synonyms | Comment | Organism |
---|---|---|
2,4-DAPDH | - |
Fervidobacterium nodosum |
2,4-diaminopentanoate dehydrogenase | - |
Fervidobacterium nodosum |
Fnod_1646 | - |
Fervidobacterium nodosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Fervidobacterium nodosum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | more than 50% activity between 70 and 100°C | Fervidobacterium nodosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 100 | the half-denaturation time at 90°C and 100°C is 38 min and 2 min, respectively | Fervidobacterium nodosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
2,5-Diaminohexanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum | |
57 | - |
NADP+ | at pH 8.5 and 55°C | Fervidobacterium nodosum | |
65 | - |
NAD+ | at pH 8.5 and 55°C | Fervidobacterium nodosum | |
65 | - |
(2R,4S)-2,4-diaminopentanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Fervidobacterium nodosum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | the enzyme shows more than 70% of maximum activity in the pH range of 9.0-10.0 | Fervidobacterium nodosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the Km value for NAD+ is about 40 times lower than that for NADP+ | Fervidobacterium nodosum | |
NADP+ | - |
Fervidobacterium nodosum | |
pyridoxal 5'-phosphate | - |
Fervidobacterium nodosum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-ornithine | at pH 8.5 and 55°C | Fervidobacterium nodosum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
2,5-Diaminohexanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum | |
29 | - |
NADP+ | at pH 8.5 and 55°C | Fervidobacterium nodosum | |
330 | - |
(2R,4S)-2,4-diaminopentanoate | with NAD+ as cosubstrate, at pH 8.5 and 55°C | Fervidobacterium nodosum | |
1300 | - |
NAD+ | at pH 8.5 and 55°C | Fervidobacterium nodosum |