Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | associated to inner membrane | Gallus gallus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
T-protein, gel fuiltration | Gallus gallus |
220000 | - |
complex of P-, L-protein, gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P15505 | i.e. component P-protein, glycine dehydrogenase, cf. EC 1.4.4.2 | - |
Purification (Comment) | Organism |
---|---|
isolation from mitochondria by ammunium sulfate fractionation and gel filtration | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH | - |
Gallus gallus | glycine + tetrahydrofolate + NAD+ | - |
r | |
glycine + tetrahydrofolate + NAD+ | - |
Gallus gallus | 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 220000, complex of P-, L-protein, x * 40000, T-protein, gel filtration | Gallus gallus |
General Information | Comment | Organism |
---|---|---|
physiological function | the reversible glycine cleavage system in liver mitochondria involves four enzyme proteins designated as P-protein (a pyridoxal phosphate requiring protein), H-protein (a hydrogen carrier protein), L-protein (exhibiting a lipoamide dehydrogenase activity) and T-protein (a H4-folate requiring protein). All three protein fractions obtained during purification are essential for the overall reactions of glycine cleavage and glycine synthesis, while only P-, L-protein and H-protein are required for the glycine-14CO2 exchange | Gallus gallus |