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Literature summary for 1.4.1.27 extracted from

  • Hiraga, K.; Kochi, H.; Motokawa, Y.; Kikuchi, G.
    Enzyme complex nature of the reversible glycine cleavage system of cock liver mitochondria (1972), J. Biochem., 72, 1285-1289 .
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion associated to inner membrane Gallus gallus 5739
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
T-protein, gel fuiltration Gallus gallus
220000
-
complex of P-, L-protein, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus P15505 i.e. component P-protein, glycine dehydrogenase, cf. EC 1.4.4.2
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Purification (Commentary)

Purification (Comment) Organism
isolation from mitochondria by ammunium sulfate fractionation and gel filtration Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
Gallus gallus glycine + tetrahydrofolate + NAD+
-
r
glycine + tetrahydrofolate + NAD+
-
Gallus gallus 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
r

Subunits

Subunits Comment Organism
multimer x * 220000, complex of P-, L-protein, x * 40000, T-protein, gel filtration Gallus gallus

General Information

General Information Comment Organism
physiological function the reversible glycine cleavage system in liver mitochondria involves four enzyme proteins designated as P-protein (a pyridoxal phosphate requiring protein), H-protein (a hydrogen carrier protein), L-protein (exhibiting a lipoamide dehydrogenase activity) and T-protein (a H4-folate requiring protein). All three protein fractions obtained during purification are essential for the overall reactions of glycine cleavage and glycine synthesis, while only P-, L-protein and H-protein are required for the glycine-14CO2 exchange Gallus gallus