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Literature summary for 1.4.1.28 extracted from

  • Itoh, N.; Yachi, C.; Kudome, T.
    Determining a novel NAD+-dependent amine dehydrogenase with a broad substrate range from Streptomyces ═irginiae IFO 12827 purification and characterization (2000), J. Mol. Catal. B, 10, 281-290 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
NADH pH 9, 25┬░C Streptomyces virginiae
0.022
-
NADH pH 7, 25┬░C Streptomyces virginiae
0.84
-
NAD+ pH 8, 25┬░C Streptomyces virginiae
0.84
-
NAD+ pH 9, 25┬░C Streptomyces virginiae
2.2
-
dihydroxyacetone pH 9, 25┬░C Streptomyces virginiae
2.2
-
dihydroxyacetone pH 7, 25┬░C Streptomyces virginiae
4
-
serinol pH 8, 25┬░C Streptomyces virginiae
4
-
serinol pH 9, 25┬░C Streptomyces virginiae
26.5
-
NH3 pH 9, 25┬░C Streptomyces virginiae
26.5
-
NH3 pH 7, 25┬░C Streptomyces virginiae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Streptomyces virginiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration Streptomyces virginiae

Organism

Organism UniProt Comment Textmining
Streptomyces virginiae
-
-
-
Streptomyces virginiae IFO 12827
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from cell-free extract by sequential column chromatography Streptomyces virginiae
from the cell-free extract by sequential column chromatography Streptomyces virginiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenethylamine + H2O + NAD+
-
Streptomyces virginiae acetophenone + NH3 + NADH + H+
-
r
(R)-1-phenethylamine + NAD+ + H2O
-
Streptomyces virginiae phenylacetaldehyde + NH3 + NADH + H+
-
r
(R)-1-phenethylamine + NAD+ + H2O
-
Streptomyces virginiae IFO 12827 phenylacetaldehyde + NH3 + NADH + H+
-
r
(R)-2-aminoheptane + NAD+ + H2O
-
Streptomyces virginiae 2-oxoheptane + NH3 + NADH + H+
-
r
(R)-2-aminoheptane + NAD+ + H2O
-
Streptomyces virginiae IFO 12827 2-oxoheptane + NH3 + NADH + H+
-
r
(R)-2-butylamine + NAD+ + H2O
-
Streptomyces virginiae 2-oxobutane + NH3 + NADH + H+
-
r
(R)-2-butylamine + NAD+ + H2O
-
Streptomyces virginiae IFO 12827 2-oxobutane + NH3 + NADH + H+
-
r
(R,S)-2-amino-1-pentanol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-pentan-1-ol + NH3 + NADH + H+
-
r
(R,S)-2-aminopentan-1-ol + H2O + NAD+
-
Streptomyces virginiae 2-oxopentan-1-ol + NH3 + NADH + H+
-
r
(S)-1-phenethylamine + H2O + NAD+
-
Streptomyces virginiae acetophenone + NH3 + NADH + H+
-
r
(S)-1-phenethylamine + NAD+ + H2O
-
Streptomyces virginiae phenylacetaldehyde + NH3 + NADH + H+
-
r
(S)-2-amino-1-butanol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-butan-1-ol + NH3 + NADH + H+
-
r
(S)-2-amino-3-methyl-1-butanol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-3-methylbutan-1-ol + NH3 + NADH + H+
-
r
(S)-2-amino-3-methylbutan-1-ol + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxobutan-1-ol + NH3 + NADH + H+
-
r
(S)-2-aminobutan-1-ol + H2O + NAD+
-
Streptomyces virginiae 2-oxobutan-1-ol + NH3 + NADH + H+
-
r
(S)-2-aminoheptane + NAD+ + H2O
-
Streptomyces virginiae 2-oxoheptane + NH3 + NADH + H+
-
r
(S)-2-butylamine + H2O + NAD+
-
Streptomyces virginiae 2-oxobutane + NH3 + NADH + H+
-
r
(S)-2-butylamine + NAD+ + H2O
-
Streptomyces virginiae 2-oxobutane + NH3 + NADH + H+
-
r
(S)-isoleucinol + H2O + NAD+
-
Streptomyces virginiae ? + NH3 + NADH + H+
-
r
(S)-isoleucinol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-3-methylpentan-1-ol + NH3 + NADH + H+
-
r
(S)-leucinol + H2O + NAD+
-
Streptomyces virginiae ? + NH3 + NADH + H+
-
r
(S)-leucinol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-5-methylpentan-1-ol + NH3 + NADH + H+
-
r
(S)-methioninol + H2O + NAD+
-
Streptomyces virginiae ? + NH3 + NADH + H+
-
r
(S)-methioninol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-3-methylpentan-1-ol + NH3 + NADH + H+
-
r
2-amino-1-propanol + H2O + NAD+
-
Streptomyces virginiae 1-hydroxyacetone + NH3 + NADH + H+
-
r
2-aminocyclohexanol + NAD+ + H2O
-
Streptomyces virginiae 2-oxo-cyclohexanol + NH3 + NADH + H+
-
r
2-aminopentane + H2O + NAD+
-
Streptomyces virginiae 2-oxopentane + NH3 + NADH + H+
-
r
2-aminopentane + NAD+ + H2O
-
Streptomyces virginiae 2-oxopentane + NH3 + NADH + H+
-
r
2-oxopropanoate + NH3 + NADH + H+
-
Streptomyces virginiae D,L-alanine + H2O + NAD+
-
r
3-acetyl-1-propanol + NH3 + NADH + H+
-
Streptomyces virginiae 3-aminobutan-1-ol + H2O + NAD+
-
r
3-aminopentane + H2O + NAD+
-
Streptomyces virginiae 3-oxopentane + NH3 + NADH + H+
-
r
3-aminopentane + NAD+ + H2O
-
Streptomyces virginiae 3-oxopentane + NH3 + NADH + H+
-
r
3-hydroxy-2-butanone + NH3 + NADH + H+
-
Streptomyces virginiae 3-amino-butan-2-ol + NAD+ + H2O
-
r
3-hydroxy-2-butanone + NH3 + NADH + H+
-
Streptomyces virginiae 3-aminobutan-2-ol + H2O + NAD+
-
r
4-hydroxy-2-butanone + NH3 + NADH + H+
-
Streptomyces virginiae 3-amino-butan-1-ol + NAD+ + H2O
-
r
4-hydroxy-2-butanone + NH3 + NADH + H+
-
Streptomyces virginiae 3-aminobutan-1-ol + H2O + NAD+
-
r
4-hydroxyhexan-3-one + NH3 + NADH + H+
-
Streptomyces virginiae 4-aminohexanan-3-ol + NAD+ + H2O
-
r
4-phenylbutan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 4-phenylbutan-2-amine + NAD+ + H2O
-
r
5-hydroxyoctan-4-one + NH3 + NADH + H+
-
Streptomyces virginiae 5-aminooctan-4-ol + NAD+ + H2O
-
r
5-hydroxypentan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 5-aminopentan-1-ol + H2O + NAD+
-
r
acetaldehyde + NH3 + NADH + H+
-
Streptomyces virginiae ethylamine + NAD+ + H2O
-
r
acetaldehyde + NH3 + NADH + H+
-
Streptomyces virginiae ethylamine + H2O + NAD+
-
r
acetophenone + NH3 + NADH + H+
-
Streptomyces virginiae 1-phenylethan-1-amine + NAD+ + H2O
-
r
aspartate + H2O + NAD+
-
Streptomyces virginiae ? + NH3 + NADH + H+
-
r
benzaldehyde + NH3 + NADH + H+
-
Streptomyces virginiae benzylamine + H2O + NAD+
-
r
benzaldehyde + NH3 + NADH + H+
-
Streptomyces virginiae benzylamine + NAD+ + H2O
-
r
benzylamine + H2O + NAD+
-
Streptomyces virginiae benzaldehyde + NH3 + NADH + H+
-
r
benzylamine + NAD+ + H2O
-
Streptomyces virginiae benzaldehyde + NH3 + NADH + H+
-
r
butan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminobutane + NAD+ + H2O
-
r
butan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminobutane + H2O + NAD+
-
r
butanal + NH3 + NADH + H+
-
Streptomyces virginiae n-butylamine + NAD+ + H2O
-
r
butanal + NH3 + NADH + H+
-
Streptomyces virginiae butylamine + H2O + NAD+
-
r
D-alanine + H2O + NAD+
-
Streptomyces virginiae 2-oxopropanoate + NH3 + NADH + H+
-
r
D-alanine + NAD+ + H2O
-
Streptomyces virginiae 2-oxopropanoate + NH3 + NADH + H+
-
r
D-aspartate + H2O + NAD+
-
Streptomyces virginiae 2-oxobutanedioate + NH3 + NADH + H+
-
r
D-glutamate + H2O + NAD+
-
Streptomyces virginiae 2-oxopentanedioate + NH3 + NADH + H+
-
r
D-glutamate + NAD+ + H2O
-
Streptomyces virginiae 2-oxopentanedioic acid + NH3 + NADH + H+
-
r
D-serine + NAD+ + H2O
-
Streptomyces virginiae 3-hydroxy-2-oxopropanoate + NH3 + NADH + H+
-
r
dihydroxyacetone + NH3 + NADH + H+
-
Streptomyces virginiae serinol + NAD+ + H2O
-
r
dihydroxyacetone + NH3 + NADH + H+
-
Streptomyces virginiae serinol + H2O + NAD+
-
?
ethylamine + NAD+ + H2O
-
Streptomyces virginiae acetaldehyde + NH3 + NADH + H+
-
r
hexan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminohexane + NAD+ + H2O
-
r
hexanal + NH3 + NADH + H+
-
Streptomyces virginiae n-hexylamine + NAD+ + H2O
-
r
hydroxyacetone + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminopropan-1-ol + NAD+ + H2O
-
r
hydroxyacetone + NH3 + NADH + H+
-
Streptomyces virginiae 1-aminopropanone + H2O + NAD+
-
r
L-alanine + H2O + NAD+
-
Streptomyces virginiae 2-oxopropanoate + NH3 + NADH + H+
-
r
L-alanine + NAD+ + H2O
-
Streptomyces virginiae 2-oxopropanoate + NH3 + NADH + H+
-
r
L-aspartate + H2O + NAD+
-
Streptomyces virginiae 2-oxobutanedioate + NH3 + NADH + H+
-
r
L-aspartate + NAD+ + H2O
-
Streptomyces virginiae 2-oxobutanedioic acid + NH3 + NADH + H+
-
r
L-glutamate + H2O + NAD+
-
Streptomyces virginiae 2-oxopentanedioate + NH3 + NADH + H+
-
r
L-glutamate + NAD+ + H2O
-
Streptomyces virginiae 2-oxopentanedioic acid + NH3 + NADH + H+
-
r
L-serine + NAD+ + H2O
-
Streptomyces virginiae 2-oxopentanedioic acid + NH3 + NADH + H+
-
r
methylamin + NAD+ + H2O
-
Streptomyces virginiae formaldehyde + NH3 + NADH + H+
-
r
additional information enzyme catalyzes the reversible oxidative deaminations of amines and also amino alcohols and amino acids Streptomyces virginiae ?
-
-
additional information catalyzes the reversible oxidative deaminations of amines but also amino alcohols and amino acids Streptomyces virginiae ?
-
-
additional information enzyme catalyzes the reversible oxidative deaminations of amines and also amino alcohols and amino acids Streptomyces virginiae IFO 12827 ?
-
-
additional information catalyzes the reversible oxidative deaminations of amines but also amino alcohols and amino acids Streptomyces virginiae IFO 12827 ?
-
-
n-butylamine + NAD+ + H2O
-
Streptomyces virginiae butanal + NH3 + NADH + H+
-
r
n-hexylamine + NAD+ + H2O
-
Streptomyces virginiae hexanal + NH3 + NADH + H+
-
r
n-octylamine + NAD+ + H2O
-
Streptomyces virginiae octanal + NH3 + NADH + H+
-
r
n-propylamine + NAD+ + H2O
-
Streptomyces virginiae propanal + NH3 + NADH + H+
-
r
pentan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminopentane + NAD+ + H2O
-
r
pentan-2-one + NH3 + NADH + H+
-
Streptomyces virginiae 2-aminopentane + H2O + NAD+
-
r
phenethylamine + H2O + NAD+
-
Streptomyces virginiae acetophenone + NH3 + NADH + H+
-
r
propionaldehyde + NH3 + NADH + H+
-
Streptomyces virginiae n-propylamine + NAD+ + H2O
-
r
serinol + H2O + NAD+
-
Streptomyces virginiae dihydroxyacetone + NH3 + NADH + H+
-
r
serinol + NAD+ + H2O
-
Streptomyces virginiae dihydroxyacetone + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Streptomyces virginiae

Synonyms

Synonyms Comment Organism
AMDH
-
Streptomyces virginiae
NAD+-dependent amine dehydrogenase
-
Streptomyces virginiae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Streptomyces virginiae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30
-
30 min, pH 7, 55% residual activity Streptomyces virginiae
30
-
30 min, 55% residual ativity Streptomyces virginiae
40
-
30 min, pH 7, 25% residual activity Streptomyces virginiae
40
-
30 min, 25% residual ativity Streptomyces virginiae
45
-
30 min, pH 7, 0% residual activity Streptomyces virginiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 amination reaction Streptomyces virginiae
6.5 7 aminating reaction Streptomyces virginiae
10
-
deaminating reaction Streptomyces virginiae

Cofactor

Cofactor Comment Organism Structure
additional information enzyme does not require artificial electron acceptors such as phenazine methosulfate Streptomyces virginiae
NAD+ enzyme is dependent on NAD+ or NADH Streptomyces virginiae
NAD+ dependent on NAD+ or NADH Streptomyces virginiae
NADH enzyme is dependent on NAD+ or NADH Streptomyces virginiae
NADH dependent on NAD+ or NADH Streptomyces virginiae
NADP+ substrate serinol, 13% of the activity with NAD+ Streptomyces virginiae
NADP+ about 13% of the activity with NAD+ Streptomyces virginiae
NADPH substrate dihydroxyacetone, 8.5% of the activity with NAD+ Streptomyces virginiae
NADPH about 8.5% of the activity with NADH Streptomyces virginiae