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Literature summary for 1.4.1.28 extracted from

  • Wang,G.; Qu, G.; Li, J.K.; Ma, J.A.; Guo,J.; Miao, Y.; Sun, Z.
    Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols (2020), Catal. Sci. Technol., 10, 5945-5952 .
No PubMed abstract available

Application

Application Comment Organism
synthesis in the asymmetric amination of a series of structurally different hydroxy ketones, high stereoselectivity (above >99%) is achieved the reduction of aliphatic and aromatic compounds. The aliphatic substrates 1-hydroxy-4-methylpentan-2-one and 1-hydroxyhexan-2-one are aminated with 99% conversions Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure in complex with NADH. Residue S68 of AmDH forms a hydrogen bond with the substrate hydroxyl group, and there are also hydrophobic interactions between the substrate and the side chains of V116 and L261 Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.48
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Geobacillus stearothermophilus
2.32
-
1-hydroxybutan-2-one pH 9.0, 55┬░C Geobacillus stearothermophilus
2.42
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Sporosarcina psychrophila
3.44
-
1-hydroxybutan-2-one pH 8.5, 55┬░C Lysinibacillus sphaericus
3.64
-
1-hydroxybutan-2-one pH 8.5, 45┬░C Thermoactinomyces intermedius

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus P13154 cf. EC 1.4.1.9
-
Lysinibacillus sphaericus W7S058 cf. EC 1.4.1.9
-
Lysinibacillus sphaericus CBAM5 W7S058 cf. EC 1.4.1.9
-
Sporosarcina psychrophila I0IJU1 cf. EC 1.4.1.9
-
Thermoactinomyces intermedius Q60030 cf. EC 1.4.1.9
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-4-methylpentan-2-one + NH3 + NADH + H+
-
Geobacillus stearothermophilus 2-amino-4-methylpentan-1-ol + H2O + NAD+
-
?
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+
-
Geobacillus stearothermophilus (S)-2-aminobutan-1-ol + H2O + NAD(P)+
-
?
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+
-
Lysinibacillus sphaericus (S)-2-aminobutan-1-ol + H2O + NAD(P)+
-
?
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+
-
Sporosarcina psychrophila (S)-2-aminobutan-1-ol + H2O + NAD(P)+
-
?
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+
-
Thermoactinomyces intermedius (S)-2-aminobutan-1-ol + H2O + NAD(P)+
-
?
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+
-
Lysinibacillus sphaericus CBAM5 (S)-2-aminobutan-1-ol + H2O + NAD(P)+
-
?
1-hydroxyhexan-2-one + NH3 + NADH + H+
-
Geobacillus stearothermophilus (S)-2-aminohexan-1-ol + H2O + NAD+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
-
Thermoactinomyces intermedius
50
-
-
Geobacillus stearothermophilus
55
-
-
Geobacillus stearothermophilus
55
-
-
Lysinibacillus sphaericus
55
-
-
Sporosarcina psychrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
1-hydroxybutan-2-one pH 8.5, 45┬░C Thermoactinomyces intermedius
0.74
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Sporosarcina psychrophila
0.77
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Geobacillus stearothermophilus
1.52
-
1-hydroxybutan-2-one pH 8.5, 55┬░C Lysinibacillus sphaericus
1.79
-
1-hydroxybutan-2-one pH 9.0, 55┬░C Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Geobacillus stearothermophilus
8.5
-
-
Lysinibacillus sphaericus
8.5
-
-
Sporosarcina psychrophila
8.5
-
-
Thermoactinomyces intermedius
9
-
-
Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
1-hydroxybutan-2-one pH 8.5, 45┬░C Thermoactinomyces intermedius
0.31
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Sporosarcina psychrophila
0.44
-
1-hydroxybutan-2-one pH 8.5, 55┬░C Lysinibacillus sphaericus
0.52
-
1-hydroxybutan-2-one pH 8.5, 50┬░C Geobacillus stearothermophilus
0.71
-
1-hydroxybutan-2-one pH 9.0, 55┬░C Geobacillus stearothermophilus