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Literature summary for 1.4.1.3 extracted from

  • Tang, M.Q.; Ando, S.; Yamada, S.; Hayashi, S.
    The trypsin-catalyzed activation of glutamate dehydrogenase purified from eel liver (1992), J. Biochem., 111, 655-661.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Trypsin limited trypsin proteolyis activates the purified enzyme 8fold if the peptide is absent from the assay mixture, the native enzyme is 3fold activated if the cleaved peptide is present, activation may therefore be induced by loss of the peptide from the subunit of the native enzyme Anguilla anguilla

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
6 * 54000, SDS-PAGE Anguilla anguilla
340000
-
gel filtration Anguilla anguilla

Organism

Organism UniProt Comment Textmining
Anguilla anguilla
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification limited trypsin proteolysis removes a 39 amino acid peptide from the enzyme Anguilla anguilla

Purification (Commentary)

Purification (Comment) Organism
hydroxylapatite, DEAE-Toyopearl, GTP-Sepharose Anguilla anguilla

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Anguilla anguilla
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.53
-
-
Anguilla anguilla

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Anguilla anguilla 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 54000, SDS-PAGE Anguilla anguilla