Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | stimulation | Thunnus thynnus |
Crystallization (Comment) | Organism |
---|---|
- |
Thunnus thynnus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Thunnus thynnus | |
isophthalate | competitive vs. glutamate | Thunnus thynnus | |
pyridoxal 5'-phosphate | 0.11 mM, approx. 30% inactivation after 10 min, 0.78 mM, 80% inactivation, inactivation is completely reversed by dialysis | Thunnus thynnus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53900 | - |
6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol | Thunnus thynnus |
333000 | - |
- |
Thunnus thynnus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thunnus thynnus | - |
tuna | - |
Purification (Comment) | Organism |
---|---|
- |
Thunnus thynnus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Thunnus thynnus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.93 | - |
after 3rd crystallization | Thunnus thynnus |
Storage Stability | Organism |
---|---|
suspended in ammonium sulfate, stable for months | Thunnus thynnus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Thunnus thynnus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol | Thunnus thynnus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
glutamate | oxidation | Thunnus thynnus | |
510 | - |
2-oxoglutarate | reduction | Thunnus thynnus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | reaction rate NADH/NADPH: 15 | Thunnus thynnus | |
NADPH | reaction rate NADH/NADPH: 15 | Thunnus thynnus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
isophthalate | - |
Thunnus thynnus |