Literature summary for 1.4.1.3 extracted from

McCarthy, A.D.; Tipton, K.F.
Ox glutamate dehydrogenase. Comparison of the kinetic properties of native and proteolysed preparations (1985), Biochem. J., 230, 95-99.

Search for more literature for 1.4.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	1.5fold activation, 0.08 mM NADH as cofactor	Bos taurus
ATP	4.8fold activation, cofactor 0.08 mM NADH	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
GTP	-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	NADPH	-	Bos taurus
0.02	-	NADH	-	Bos taurus
0.36	-	2-oxoglutarate	NADH + NH4Cl	Bos taurus
0.47	-	2-oxoglutarate	NADPH + NH4Cl	Bos taurus
1	-	L-glutamate	1 mM, NAD+	Bos taurus
3	-	L-glutamate	1 mM, NADP+	Bos taurus
25	-	L-glutamate	0.004 mM, NAD+	Bos taurus
44	-	NH3	NADPH + 2-oxoglutarate	Bos taurus
50	-	NH3	NADH + 2-oxoglutarate	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-
liver	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NADH + NH3	-	Bos taurus	L-glutamate + NAD+ + H2O	-	?
2-oxoglutarate + NH3 + NADH + H+	NH4Cl used in enzyme assay	Bos taurus	L-glutamate + H2O + NAD+	-	r
2-oxoglutarate + NH3 + NADPH + H+	NH4Cl used in enzyme assay	Bos taurus	L-glutamate + H2O + NADP+	-	r
L-glutamate + H2O + NAD(P)+	-	Bos taurus	2-oxoglutarate + NH3 + NAD(P)H	-	r

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0065	-	GTP	1 mM NAD+ or 80 mM NADH	Bos taurus
0.009	-	GTP	0.08 mM NADH	Bos taurus
0.012	-	GTP	0.016 mM NADH	Bos taurus
0.018	-	GTP	1 mM NADP+	Bos taurus

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Release 2023.1 (January 2023)