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Literature summary for 1.4.1.3 extracted from

  • Thatcher, B.J.; Storey, K.B.
    Glutamate dehydrogenase from liver of euthermic and hibernating Richardson's ground squirrels: Evidence for two distinct enzyme forms (2001), Biochem. Cell Biol., 79, 11-19.
    View publication on PubMed
Search for more literature for 1.4.1.3

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
 Bos taurus
ADP
-
 Urocitellus richardsonii
AMP
-
 Bos taurus
AMP
-
 Urocitellus richardsonii
KCl activates at 5°C and 37°C Urocitellus richardsonii
leucine activates at 5°C only Urocitellus richardsonii
NaCl activates at 37°C only Urocitellus richardsonii

Inhibitors

Inhibitors Comment Organism Structure
ATP strong inhibition at 37°C, no inhibition at 5°C Bos taurus
ATP strong inhibition at 37°C, no inhibition at 5°C Urocitellus richardsonii
GTP
-
 Bos taurus
GTP strong inhibition at 37°C, weak inhibition at 5°C Urocitellus richardsonii
additional information complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C Bos taurus
additional information complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C Urocitellus richardsonii
NaCl
-
 Bos taurus
Sodium acetate at 5°C only Urocitellus richardsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-oxoglutarate euthermic animal, assay at 37°C Urocitellus richardsonii
0.25
-
 glutamate hibernating animal, assay at 5°C Urocitellus richardsonii
0.43
-
 2-oxoglutarate hibernating animal, assay at 5°C Urocitellus richardsonii
0.47
-
 glutamate assay at 5°C Urocitellus richardsonii
0.5
-
 glutamate euthermic animal, assay at 5°C Urocitellus richardsonii
0.98
-
 2-oxoglutarate hibernating animal, assay at 37°C Urocitellus richardsonii
1.4
-
 2-oxoglutarate
-
 Bos taurus
1.4
-
 2-oxoglutarate assay at 37°C Urocitellus richardsonii
2.03
-
 glutamate euthermic animal, assay at 37°C Urocitellus richardsonii
2.6
-
 glutamate assay at 37°C Urocitellus richardsonii
3.66
-
 2-oxoglutarate euthermic animal, assay at 5°C Urocitellus richardsonii
4.1
-
 NH4+
-
 Bos taurus
4.1
-
 NH4+ assay at 5°C Urocitellus richardsonii
5.2
-
 glutamate hibernating animal, assay at 37°C Urocitellus richardsonii
7
-
 NH4+ euthermic animal, assay at 37°C Urocitellus richardsonii
10.5
-
 2-oxoglutarate
-
 Bos taurus
10.5
-
 2-oxoglutarate assay at 5°C Urocitellus richardsonii
12.1
-
 NH4+ hibernating animal, assay at 5°C Urocitellus richardsonii
15.8
-
 NH4+ hibernating animal, assay at 37°C Urocitellus richardsonii
17.8
-
 NH4+
-
 Bos taurus
17.8
-
 NH4+ assay at 37°C Urocitellus richardsonii
24.7
-
 NH4+ euthermic animal, assay at 5°C Urocitellus richardsonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59500
-
 6 * 59500, enzyme from both euthermic and hibernating animals Urocitellus richardsonii
320000
-
 enzyme from hibernating animals, gel filtration Urocitellus richardsonii
331000
-
 gel filtration Bos taurus
335000
-
 enzyme from euthermic animals, gel filtration Urocitellus richardsonii

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Urocitellus richardsonii
-
 Richardson's ground squirrel
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from euthermic and hibernating animals Urocitellus richardsonii

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-
liver from euthermic, 37°C body temperature, and hibernating, 5°C body temperature, animals Urocitellus richardsonii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
 enzyme from euthermic animals Urocitellus richardsonii
201
-
 enzyme from hibernating animals Urocitellus richardsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
 Bos taurus 2-oxoglutarate + NH3 + NAD(P)H
-
 r
L-glutamate + H2O + NAD(P)+
-
 Urocitellus richardsonii 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 59500, enzyme from both euthermic and hibernating animals Urocitellus richardsonii