Literature summary for 1.4.1.3 extracted from

Kim, D.W.; Eum, W.S.; Jang, S.H.; Yoon, C.S.; Kim, Y.H.; Choi, S.H.; Choi, H.S.; Kim, S.Y.; Kwon, H.Y.; Kang, J.H.; Kwon, O.s.; Cho, S.w.; Park, J.; Choi, S.Y.
Molecular gene cloning, expression, and characterization of bovine brain glutamate dehydrogenase (2003), J. Biochem. Mol. Biol., 36, 545-551.

Search for more literature for 1.4.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	increases the activity up to 2.3fold	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 by using the pET-15b expression vector containing a T7 promoter	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	0.01-0.1 mM	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	NAD+	pH 8.0, 25°C, recombinant enzyme	Bos taurus
0.11	-	NAD+	pH 8.0, 25°C, native enzyme	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57500	-	x * 57500, recombinant enzyme, SDS-PAGE	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NADH + NH3	-	Bos taurus	L-glutamate + NAD+ + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 57500, recombinant enzyme, SDS-PAGE	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bos taurus
NADH	-	Bos taurus

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Release 2023.1 (January 2023)