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Literature summary for 1.4.1.4 extracted from
- Glutamate dehydrogenase from the extremely thermophilic archaebacterial isolate AN1 (1993), Biochim. Biophys. Acta, 1202, 244-250.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | at 5 mM, 135% activation, oxidative deamination | Thermococcus sp. |  |
| MgCl2 | at 5 mM, 104% activity, oxidative deamination | Thermococcus sp. | |
| MnCl2 | at 5 mM, 250% activity, oxidative deamination | Thermococcus sp. | |
General Stability
| General Stability | Organism |
|---|---|
| 10 mM MOPS-NaOH pH 7.1 and 0.3-0.4 M NaCl is the best buffer | Thermococcus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | at 1 mM, no activity, oxidative deamination | Thermococcus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
NADP+ | oxidative deamination at 80°C | Thermococcus sp. | |
| 0.066 | - |
NADPH | reductive amination at 80°C | Thermococcus sp. | |
| 1.7 | - |
2-oxoglutarate | reductive amination at 80°C | Thermococcus sp. | |
| 9.12 | - |
L-glutamate | oxidative deamination at 80°C | Thermococcus sp. | |
| 15.5 | - |
NH4+ | reductive amination at 80°C | Thermococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
alpha4, 4 * 47000, SDS-PAGE | Thermococcus sp. |
| 204000 | - |
gel filtration | Thermococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Thermococcus sp. | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus sp. | - |
isolate AN1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 464 | - |
oxidative deamination at 80°C and pH 7.54 | Thermococcus sp. |
| 2940 | - |
reductive amination at 80°C and pH 7.54 | Thermococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Thermococcus sp. | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Thermococcus sp. | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | alpha4, 4 * 47000, SDS-PAGE | Thermococcus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
50% activity after 12.5 h in 0.1 M NaH2PO4/Na2HPO4 | Thermococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
reductive amination and oxidative deamination | Thermococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Thermococcus sp. | |
| NADPH | - |
Thermococcus sp. | |
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