Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli DH5alpha under the control of a highly constitutive expresion promoter in a pHCE system | Symbiobacterium toebii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
NADP+ | pH 9.0, 50°C | Symbiobacterium toebii | |
0.28 | - |
NADPH | pH 9.0, 50°C | Symbiobacterium toebii | |
0.368 | - |
NADH | pH 9.0, 50°C | Symbiobacterium toebii | |
3.629 | - |
NAD+ | pH 9.0, 50°C | Symbiobacterium toebii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
6 * 44000, SDS-PAGE | Symbiobacterium toebii |
263000 | - |
gel filtration | Symbiobacterium toebii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Symbiobacterium toebii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Symbiobacterium toebii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.8 | - |
pH 9.0, 50°C | Symbiobacterium toebii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Symbiobacterium toebii | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADPH | - |
Symbiobacterium toebii | L-glutamate + H2O + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 44000, SDS-PAGE | Symbiobacterium toebii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Symbiobacterium toebii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 65% of maximal activity, 70°C: about 85% of maximal activity | Symbiobacterium toebii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
20 h, stable | Symbiobacterium toebii |
40 | 55 | 30 min, stable | Symbiobacterium toebii |
80 | - |
30 min, complete loss of activity | Symbiobacterium toebii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
NAD+ | pH 9.0, 50°C | Symbiobacterium toebii | |
2136 | - |
NADP+ | pH 9.0, 50°C | Symbiobacterium toebii | |
6144 | - |
NADH | pH 9.0, 50°C | Symbiobacterium toebii | |
28650 | - |
NADPH | pH 9.0, 50°C | Symbiobacterium toebii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Symbiobacterium toebii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | pH 8.5: about 60% of maximal activity, pH 10.0: about 40% of maximal activity | Symbiobacterium toebii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the ratio of turnover-number to KM-value for NADP+ is 72679fold higher than the ratio for NAD+ | Symbiobacterium toebii | |
NADH | the ratio of turnover-number to KM-value for NADPH is 6fold higher than the ratio for NADH | Symbiobacterium toebii | |
NADP+ | preferred coenzyme. The ratio of turnover-number to KM-value for NADP+ is 72679fold higher than the ratio for NAD+ | Symbiobacterium toebii | |
NADPH | preferred coenzyme. The ratio of turnover-number to KM-value for NADPH is 6fold higher than the ratio for NADH | Symbiobacterium toebii |