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Literature summary for 1.4.1.4 extracted from

  • Choudhury, R.; Noor, S.; Varadarajalu, L.; Punekar, N.
    Delineation of an in vivo inhibitor for Aspergillus glutamate dehydrogenase (2008), Enzyme Microb. Technol., 42, 151-159.
    View publication on PubMed

Application

Application Comment Organism
molecular biology results implicate glutamate dehydrogenase and NADP-GDH in particular, as a key target of in vivo isophthalate inhibition during ammonium assimilation Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
2,4-pyridinedicarboxylate inhibits less efficiently Aspergillus niger
2-Methyleneglutarate
-
Aspergillus niger
dimethyl ester of isophthalic acid dimethyl ester of isophthalate (DMIP), but not isophthalate, inhibits Aspergillus niger growth on agar as well as in liquid culture. This is ascribed to the inability of isophthalate to enter fungal mycelia. Dimethyl ester of isophthalic acid is hydrolysed intracellularly to isophthalate. Subsequent to DMIP addition, intracellular isophthalate can be demonstrated. Addition of NH4+ to DMIP-treated Aspergillus niger mycelia results in intensive vacuolation, retraction of cytoplasm and autolysis Aspergillus niger
isophthalate potent in vitro inhibitor Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Aspergillus niger L-glutamate + NADP+
-
?

Synonyms

Synonyms Comment Organism
glutamate dehydrogenase
-
Aspergillus niger
NADP-GDH
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Aspergillus niger