Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isophthalate | - |
Acinetobacter lwoffii | |
isophthalate | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter lwoffii | - |
strain ISP4, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH | - |
Pseudomonas aeruginosa | - |
strain PP4, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH | - |
Pseudomonas aeruginosa | - |
strain PPD, when grown on isophthalate, Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD show activity of NADP-dependent GDH, while cells grown on glucose, 2fold yeast extract-tryptone broth (2YT) or glutamate show activities of both NAD-dependent GDH and NADP-GDH | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
late-exponential-phase cells are used for this study since they show a higher activity of GDH compared with early exponential-phase cells | Pseudomonas aeruginosa |
additional information | - |
late-exponential-phase cells are used for this study since they show a higher activity of GDH compared with early exponential-phase cells | Acinetobacter lwoffii |
Synonyms | Comment | Organism |
---|---|---|
GdhA | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD | Pseudomonas aeruginosa |
GDHB | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD | Pseudomonas aeruginosa |
GDHC | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of three (GDHA, GDHB and GDHC) forms of NADP-GDH in Pseudomonas aeruginosa strain PPD | Pseudomonas aeruginosa |
GDHI | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of two (GDHI and GDHII) forms of NADP-GDH in Pseudomonas aeruginosa strain PP4 | Pseudomonas aeruginosa |
GDHII | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of two (GDHI and GDHII) forms of NADP-GDH in Pseudomonas aeruginosa strain PP4 | Pseudomonas aeruginosa |
GDHP | activity staining, inhibition and thermal stability studies indicate the carbon source-dependent presence of one GDHP form of NADP-GDH in Acinetobacter lwoffii strain ISP4 | Acinetobacter lwoffii |
NADP-glutamate dehydrogenase | - |
Pseudomonas aeruginosa |
NADP-glutamate dehydrogenase | - |
Acinetobacter lwoffii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
time-dependent changes in the activity of NADP-GDH at 60°C are analysed: GDHII loses activity completely within 10 min | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pseudomonas aeruginosa |
8.5 | - |
assay at | Acinetobacter lwoffii |
General Information | Comment | Organism |
---|---|---|
physiological function | carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD | Pseudomonas aeruginosa |
physiological function | carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: GDHI from isophthalate- and mHB-grown cells retain 70% and 90% of its activity, respectively | Pseudomonas aeruginosa |
physiological function | carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: In Pseudomonas aeruginosa strain PPD, isophthalate-, glucose-, 2YT- or mHB-grown cells retain 100% of the activity of NADP-GDH, while PPD cells grown on pHB and benzoate show 25% and 40% loss of activity | Pseudomonas aeruginosa |
physiological function | carbon source-dependent modulation of different forms of NADP-GDH in bacterial strains Acinetobacter lwoffii strain ISP4, Pseudomonas aeruginosa strain PP4 and Pseudomonas strain PPD. Time-dependent changes in the activity of NADP-GDH at 60°C are analysed: isophthalate-, glucose-, 2YT- or mHB-grown cells retain 100% of the activity of NADP-GDH | Acinetobacter lwoffii |