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Literature summary for 1.4.1.4 extracted from

  • Walvekar, A.S.; Choudhury, R.; Punekar, N.S.
    Mixed disulfide formation at Cys141 leads to apparent unidirectional attenuation of Aspergillus niger NADP-glutamate dehydrogenase activity (2014), PLoS ONE, 9, e101662 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus niger

Protein Variants

Protein Variants Comment Organism
C141S reductive amination activity of C141S alone is insensitive to treatment with 2-hydroxyethyl disulfide Aspergillus niger
C415S mutant loses its reductive amination activity in a manner very similar to the native enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyethyl disulfide attenuation of reductive amination (forward) activity but a negligible effect on oxidative deamination (reverse) activity Aspergillus niger
cystine strongly and selectively inhibits the reductive amination reaction Aspergillus niger
additional information not inhibitory/activating: oxidized glutathione Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 NADPH wild-type, pH 8.0, temperature not specified in the publication Aspergillus niger
0.017
-
 NADP+ wild-type, pH 9.3, temperature not specified in the publication Aspergillus niger
0.022
-
 NADP+ wild-type inactivated by 2-hydroxyethyl disulfide, pH 9.3, temperature not specified in the publication Aspergillus niger
0.023
-
 NADPH recombinant wild-type, pH 8.0, temperature not specified in the publication Aspergillus niger
0.028
-
 NADP+ recombinant wild-type, pH 9.3, temperature not specified in the publication Aspergillus niger
0.044
-
 NADPH wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication Aspergillus niger
1.05
-
 NH3 wild-type, pH 8.0, temperature not specified in the publication Aspergillus niger
1.1
-
 NH3 recombinant wild-type, pH 8.0, temperature not specified in the publication Aspergillus niger
1.2
-
 NH3 mutant C141S, pH 8.0, temperature not specified in the publication Aspergillus niger
2.6
-
 NH3 wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication Aspergillus niger
4
-
 2-oxoglutarate mutant C141S, S0.5 value, Hill coefficient 2.3, pH 8.0, temperature not specified in the publication Aspergillus niger
4.78
-
 2-oxoglutarate wild-type, S0.5 value, Hill coefficient 3.2, pH 8.0, temperature not specified in the publication Aspergillus niger
5.7
-
 2-oxoglutarate recombinant wild-type, S0.5 value, Hill coefficient 3.5, pH 8.0, temperature not specified in the publication Aspergillus niger
15.5
-
 2-oxoglutarate mutant C415S, S0.5 value, Hill coefficient 4.9, pH 8.0, temperature not specified in the publication Aspergillus niger
25.1
-
 2-oxoglutarate wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 2.4, pH 8.0, temperature not specified in the publication Aspergillus niger
28.6
-
 L-glutamate mutant C141S, pH 9.3, temperature not specified in the publication Aspergillus niger
30.9
-
 L-glutamate recombinant wild-type, pH 9.3, temperature not specified in the publication Aspergillus niger
34.6
-
 L-glutamate wild-type, pH 9.3, temperature not specified in the publication Aspergillus niger
36.8
-
 L-glutamate mutant C415S, pH 9.3, temperature not specified in the publication Aspergillus niger
49.8
-
 L-glutamate wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 1.9, pH 9.3, temperature not specified in the publication Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger B6V7E4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
 Aspergillus niger 2-oxoglutarate + NH3 + NADPH + H+
-
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