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Literature summary for 1.4.1.9 extracted from

  • Ohshima, T.; Nagata, S.; Soda, K.
    Purification and characterization of thermostable leucine dehydrogenase from Bacillus stearothermophilus (1985), Arch. Microbiol., 141, 407-411.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu(CH3COO)2 1 mM, 20% inhibition Geobacillus stearothermophilus
Cu2+ 1 mM CuCl2, 22% inhibition Geobacillus stearothermophilus
HgCl2 0.01 M, 65% inhibition Geobacillus stearothermophilus
Pb(CH3COO)2 1 mM, 20% inhibition Geobacillus stearothermophilus
PCMB 0.01 M, 50% inhibition Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
6 * 49000, SDS-PAGE Geobacillus stearothermophilus
300000
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 month Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ile + H2O + NAD+ 73% of the activity with L-Leu Geobacillus stearothermophilus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Geobacillus stearothermophilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Met + H2O + NAD+ no activity Geobacillus stearothermophilus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
?
L-Val + H2O + NAD+ 98% of the activity with L-Leu Geobacillus stearothermophilus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 49000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
68 70 oxidative amination of L-Leu Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: 37% of maximal activity, 80°C: 55% of maximal activity, oxidative amination of L-Leu Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
20 min, enzyme retains full activity Geobacillus stearothermophilus
80
-
5 min, substantial loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
reductive amination of 3-methyl-2-oxobutanoate Geobacillus stearothermophilus
9.7
-
reductive amination of 4-methyl-2-oxopentanoate Geobacillus stearothermophilus
11
-
oxidative deamination of L-Leu Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 55°C, 5 min, stable Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Geobacillus stearothermophilus
additional information no activity with NADP+ Geobacillus stearothermophilus
NAD+
-
Geobacillus stearothermophilus
NADH
-
Geobacillus stearothermophilus