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Literature summary for 1.4.1.9 extracted from

  • Chiriac, M.; Lupan, I.; Bucurenci, N.; Popescu, O.; Palibroda, N.
    Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system (2008), J. Labelled Compd. Radiopharm., 51, 171-174.
No PubMed abstract available
Search for more literature for 1.4.1.9

Application

Application Comment Organism
biotechnology an efficient stereospecific enzymatic synthesis of L-valine, L-leucine, L-norvaline, L-norleucine and L-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1-10 g) Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P13154
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-keto beta-methylvalerate + NH3 + NADH + H+ yield: 95% Geobacillus stearothermophilus L-isoleucine + H2O + NAD+
-
 ?
alpha-ketocaproate + NH3 + NADH + H+ yield: 80% Geobacillus stearothermophilus L-norleucine + H2O + NAD+
-
 ?
alpha-ketoisocaproate + NH3 + NADH + H+ yield: 92.5% Geobacillus stearothermophilus L-leucine + H2O + NAD+
-
 ?
alpha-ketoisovalerate + NH3 + NADH + H+ yield: 90% Geobacillus stearothermophilus L-valine + H2O + NAD+
-
 ?
alpha-ketovalerate + NH3 + NADH + H+ yield: 92% Geobacillus stearothermophilus L-norvaline + H2O + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
LeuDH
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Geobacillus stearothermophilus