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Literature summary for 1.4.1.9 extracted from

  • Lu, J.; Zhang, Y.; Sun, D.; Jiang, W.; Wang, S.; Fang, B.
    The development of leucine dehydrogenase and formate dehydrogenase bifunctional enzyme cascade improves the biosynthsis of L-tert-teucine (2016), Appl. Biochem. Biotechnol., 180, 1180-1195 .
    View publication on PubMed
Search for more literature for 1.4.1.9

Application

Application Comment Organism
synthesis formation of a bifunctional enzyme complex consisting of leucine dehydrogenase (LDH) and formate dehydrogenase from Candida boidinii via a miniscaffoldin for production of L-tert-leucine. Ninety-one grams of L-tert-leucine per liter with an enantiomeric purity of 99% e.e. can be obtained Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 NADH enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus
0.248
-
 NADH His-tagged enzyme, pH 9.0, 25°C Bacillus cereus
153.6
-
 3,3-dimethyl-2-oxobutanoate enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus
217.7
-
 3,3-dimethyl-2-oxobutanoate His-tagged enzyme, pH 9.0, 25°C Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
 Bacillus cereus L-tert-Leu + H2O + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 NADH enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus
5.4
-
 NADH His-tagged enzyme, pH 9.0, 25°C Bacillus cereus
19.4
-
 3,3-dimethyl-2-oxobutanoate His-tagged enzyme, pH 9.0, 25°C Bacillus cereus
40.4
-
 3,3-dimethyl-2-oxobutanoate enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
 3,3-dimethyl-2-oxobutanoate His-tagged enzyme, pH 9.0, 25°C Bacillus cereus
0.26
-
 3,3-dimethyl-2-oxobutanoate enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus
21.8
-
 NADH His-tagged enzyme, pH 9.0, 25°C Bacillus cereus
51.3
-
 NADH enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C Bacillus cereus