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Literature summary for 1.4.1.9 extracted from

  • Zhu, W.; Li, Y.; Jia, H.; Wei, P.; Zhou, H.; Jiang, M.
    Expression, purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari (2016), Biotechnol. Lett., 38, 855-861 .
    View publication on PubMed
Search for more literature for 1.4.1.9

Inhibitors

Inhibitors Comment Organism Structure
KCl 0.5 M, specific activity 133 U/mg Laceyella sacchari
NaCl 0.5 M, specific activity 152 U/mg Laceyella sacchari

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
 NAD+ pH 10.5, 25°C Laceyella sacchari
1.4
-
 L-leucine pH 10.5, 25°C Laceyella sacchari

Organism

Organism UniProt Comment Textmining
Laceyella sacchari
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
133
-
 substrate L-leucine, presence of 0.5 M KCl, pH 10.5, 25°C Laceyella sacchari
152
-
 substrate L-leucine, presence of 0.5 M NaCl, pH 10.5, 25°C Laceyella sacchari
183
-
 substrate L-leucine, pH 10.5, 25°C Laceyella sacchari
327
-
 substrate L-leucine, pH 10.5, 60°C Laceyella sacchari

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + H2O + NAD+ 69% of the activity with L-leucine Laceyella sacchari 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
L-leucine + H2O + NAD+
-
 Laceyella sacchari 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
L-lysine + H2O + NAD+ 69% of the activity with L-leucine Laceyella sacchari 6-amino-2-oxohexanoate + NH3 + NADH + H+
-
 ?
L-valine + H2O + NAD+ 69% of the activity with L-leucine Laceyella sacchari 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 41030, calculated Laceyella sacchari
More catalytic triad consists of residues Lys 68, Lys 80 and Asp 115 Laceyella sacchari

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Laceyella sacchari

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life 2.7 h Laceyella sacchari
70
-
 half-life 1.5 h Laceyella sacchari

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Laceyella sacchari

pH Range

pH Minimum pH Maximum Comment Organism
9.5 11
-
 Laceyella sacchari

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 stable for at least 10 h Laceyella sacchari