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Literature summary for 1.4.2.1 extracted from

  • Sanders, H.K.; Becker, G.E.; Nason, A.
    Glycine-cytochrome c reductase from Nitrobacter agilis (1972), J. Biol. Chem., 247, 2015-2025.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 55% inhibition at 1 mM Nitrobacter winogradskyi
cytochrome c above 0.15 mM Nitrobacter winogradskyi
diphosphate buffer, 10 mM Nitrobacter winogradskyi
EDTA 64% inhibition at 7.1 mM Nitrobacter winogradskyi
hydrazine
-
Nitrobacter winogradskyi
hydroxylamine
-
Nitrobacter winogradskyi
KCl 80% inhibition at 75 mM Nitrobacter winogradskyi
KCN 47% inhibition at 7.1 mM Nitrobacter winogradskyi
KNO3 80% inhibition at 75 mM Nitrobacter winogradskyi
MgCl2 80% inhibition at 40 mM Nitrobacter winogradskyi
NaCl 80% inhibition at 75 mM Nitrobacter winogradskyi
phosphate buffer, 100 mM Nitrobacter winogradskyi
pyridoxal 5'-phosphate up to 80% inhibition at 0.2-2 mM due to reaction with glycine Nitrobacter winogradskyi
pyruvate 70% inhibition at 10 mM and 20% inhibition at 3 mM Nitrobacter winogradskyi
Quinacrine hydrochloride 0.3-1.0 mM Nitrobacter winogradskyi
Sodium azide 10% inhibition at 2 mM Nitrobacter winogradskyi
sodium barbital buffer, 100 mM, pH 8.0 Nitrobacter winogradskyi
Tris buffer, 100 mM, pH 8.0 Nitrobacter winogradskyi
Urea 30% inhibition at 50 mM Nitrobacter winogradskyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
ferricytochrome c
-
Nitrobacter winogradskyi
0.8
-
glycine
-
Nitrobacter winogradskyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
gel filtration, sucrose density gradient centrifugation Nitrobacter winogradskyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycine + ferricytochrome c Nitrobacter winogradskyi
-
glyoxylate + ferrocytochrome c + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Nitrobacter winogradskyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
13.1
-
-
Nitrobacter winogradskyi

Storage Stability

Storage Stability Organism
4┬░C, 4-10 weeks, 30-50% loss of activity Nitrobacter winogradskyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + ferricytochrome c
-
Nitrobacter winogradskyi glyoxylate + ferrocytochrome c + NH3
-
ir
glycine + ferricytochrome c highly specific Nitrobacter winogradskyi glyoxylate + ferrocytochrome c + NH3
-
ir
glycine + H2O + ferricytochrome c
-
Nitrobacter winogradskyi glyoxylate + NH3 + ferrocytochrome c + H+
-
r

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
4
-
4-10 weeks, 30-50% loss of activity Nitrobacter winogradskyi
70
-
5 min: 75% loss of activity, 2 min: 50% loss of activity Nitrobacter winogradskyi
80
-
2 min: 100% loss of activity Nitrobacter winogradskyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1 6.9 in potassium phosphate or diphosphate buffer Nitrobacter winogradskyi