Cloned (Comment) | Organism |
---|---|
expression of GST-tagged, N-terminally His-tagged, and S-tagged DDO | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
R237A | site-directed mutagenesis, active site mutant, shows reduced binding of inhibitor thiolactomycin compared to the wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R)-4-hydroxy-3,5-dimethyl-5-[(1E)-2-methyl-1,3-butadienyl]-2(5H)-thiophenone | i.e. thiolactomycin, active site-directed DDO inhibitor, competitive inhibition, competes with both the substrate and the coenzyme FAD; i.e. thiolactomycin, competitive inhibition, competes with both the substrate and the coenzyme FAD | Homo sapiens | |
(5R)-4-hydroxy-3,5-dimethyl-5-[(1E)-2-methyl-1,3-butadienyl]-2(5H)-thiophenone | i.e. thiolactomycin, active site-directed DDO inhibitor involving binding via Arg237, mixed type of inhibition, competes with both the substrate and the coenzyme FAD, structural models of mouse DDO/TLM complexes; i.e. thiolactomycin, competitive inhibition, competes with both the substrate and the coenzyme FAD | Mus musculus | |
malonate | - |
Homo sapiens | |
malonate | competitive inhibition | Mus musculus | |
meso-tartrate | - |
Homo sapiens | |
meso-tartrate | competitive inhibition | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-aspartate + H2O + O2 | Mus musculus | - |
oxaloacetate + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | Homo sapiens | - |
oxaloacetate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus | - |
male ICR mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-aspartate + H2O + O2 | - |
Mus musculus | oxaloacetate + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | - |
Homo sapiens | oxaloacetate + NH3 + H2O2 | - |
? | |
additional information | structure-function relationships of DDO, overview | Mus musculus | ? | - |
? | |
additional information | structure-function relationships of DDO, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Mus musculus |
DAO | - |
Homo sapiens |
DDO | - |
Mus musculus |
DDO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mus musculus | |
FAD | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Mus musculus | |
0.66 | - |
(5R)-4-hydroxy-3,5-dimethyl-5-[(1E)-2-methyl-1,3-butadienyl]-2(5H)-thiophenone | pH 8.3, 37°C | Mus musculus | |
1.59 | - |
malonate | pH 8.3, 37°C | Mus musculus | |
1.94 | - |
meso-tartrate | pH 8.3, 37°C | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.86 | - |
pH 8.3, 37°C | Mus musculus | (5R)-4-hydroxy-3,5-dimethyl-5-[(1E)-2-methyl-1,3-butadienyl]-2(5H)-thiophenone | |
5.3 | - |
pH 8.3, 37°C | Mus musculus | malonate | |
8.71 | - |
pH 8.3, 37°C | Mus musculus | meso-tartrate |