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Literature summary for 1.4.3.10 extracted from

  • Kamathewatta, N.J.B.; Deay, D.O.; Karaca, B.T.; Seibold, S.; Nguyen, T.M.; Tomas, B.; Richter, M.L.; Berrie, C.L.; Tamerler, C.
    Self-immobilized putrescine oxidase biocatalyst system engineered with a metal binding peptide (2020), Langmuir, 36, 11908-11917 .
    View publication on PubMed
Search for more literature for 1.4.3.10

Application

Application Comment Organism
diagnostics diagnostic tool for the detection of biogenic amines such as putrescine and cadaverine. In body fluids, increased levels of these biogenic amines reflect tumor growth rates and thus can be used as tumor biomarkers. The enzyme is also used to monitor food freshness by detecting polyamines in spoiling food. The use of PutOx engineered with a peptide tag (self-assembled enzyme PutOx-AuBP bound to gold nanoparticles) offers a precise assembly while securing the enzyme function in a single step and can be adapted easily as a promising strategy for designing immobilized enzyme biocatalysts in multicomponent systems Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 putrescine pH 8.0, 25°C Rhodococcus erythropolis
0.0086
-
 putrescine pH 8.0, 25°C, self-assembled enzyme PutOx-AuBP bound to gold nanoparticles Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
-
 Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
putrescine + O2 + H2O Rhodococcus erythropolis
-
 4-aminobutanal + NH3 + H2O2
-
 ?
putrescine + O2 + H2O Rhodococcus erythropolis NCIMB 11540
-
 4-aminobutanal + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis B0F9F6
-
-
Rhodococcus erythropolis NCIMB 11540 B0F9F6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
putrescine + O2 + H2O
-
 Rhodococcus erythropolis 4-aminobutanal + NH3 + H2O2
-
 ?
putrescine + O2 + H2O
-
 Rhodococcus erythropolis NCIMB 11540 4-aminobutanal + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
PutOx
-
 Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.5
-
 putrescine pH 8.0, 25°C, self-assembled enzyme PutOx-AuBP bound to gold nanoparticles Rhodococcus erythropolis
26.4
-
 putrescine pH 8.0, 25°C Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FAD the isolated enzyme appears to include only one noncovalently bound FAD per dimeric protein Rhodococcus erythropolis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2965
-
 putrescine pH 8.0, 25°C, self-assembled enzyme PutOx-AuBP bound to gold nanoparticles Rhodococcus erythropolis
3200
-
 putrescine pH 8.0, 25°C Rhodococcus erythropolis