Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces mobaraensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
L-glutamate | at pH 6.0 and 35°C | Streptomyces mobaraensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+, Li+, Mn2+, Mg2+ and Ca2+ at 5 mM have no detectable effect on the enzyme activity | Streptomyces mobaraensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | Streptomyces mobaraensis | - |
2-oxoglutarate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | A0A1S6XWY4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | - |
Streptomyces mobaraensis | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamine + O2 + H2O | 3.6% activity compared to L-glutamate | Streptomyces mobaraensis | 2-oxoglutaramate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Streptomyces mobaraensis |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces mobaraensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces mobaraensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | more than 80% activity between 20 and 50°C | Streptomyces mobaraensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | the enzyme remains stable between 20 and 37°C for 24 h | Streptomyces mobaraensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptomyces mobaraensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the recombinant enzyme retains about 90% of its initial activity after being incubated from pH 4.0 to 6.0 at 4°C for 24 h | Streptomyces mobaraensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces mobaraensis |