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Literature summary for 1.4.3.11 extracted from

  • Wu, J.; Fan, X.; Liu, J.; Luo, Q.; Xu, J.; Chen, X.
    Promoter engineering of cascade biocatalysis for alpha-ketoglutaric acid production by coexpressing L-glutamate oxidase and catalase (2018), Appl. Microbiol. Biotechnol., 102, 4755-4764 .
    View publication on PubMed

Application

Application Comment Organism
biotechnology to simplify technological processes and reduce production costs, cascade biocatalysis for 2-oxoglutarate production is constructed by simultaneously expressing L-glutamate oxidase (LGOX) from Streptomyces viridosporus and KatG from Escherichia coli W3110 in Escherichia coli BL21 (DE3). In vivo cascade biocatalysis is constructed and optimized by promoter engineering to finely control the coexpression of LGOX and KatG, thus resulting in a significant increase in 2-oxoglutarate concentration and its conversion rate with no catalase addition Streptomyces viridosporus
synthesis to simplify technological processes and reduce production costs, cascade biocatalysis for 2-oxoglutarate production is constructed by simultaneously expressing L-glutamate oxidase (LGOX) from Streptomyces viridosporus and KatG from Escherichia coli W3110 in Escherichia coli BL21 (DE3). In vivo cascade biocatalysis is constructed and optimized by promoter engineering to finely control the coexpression of LGOX and KatG, thus resulting in a significant increase in 2-oxoglutarate concentration and its conversion rate with no catalase addition Streptomyces viridosporus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces viridosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3
-
L-glutamate pH 6.5, 30°C Streptomyces viridosporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces viridosporus
-
2-oxoglutarate + NH3 + H2O2
-
?
L-glutamate + O2 + H2O Streptomyces viridosporus ATCC 14672
-
2-oxoglutarate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces viridosporus D6A5I3
-
-
Streptomyces viridosporus ATCC 14672 D6A5I3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces viridosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
Streptomyces viridosporus 2-oxoglutarate + NH3 + H2O2
-
?
L-glutamate + O2 + H2O
-
Streptomyces viridosporus ATCC 14672 2-oxoglutarate + NH3 + H2O2
-
?

Synonyms

Synonyms Comment Organism
LGOX
-
Streptomyces viridosporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
L-glutamate pH 6.5, 30°C Streptomyces viridosporus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0033
-
L-glutamate pH 6.5, 30°C Streptomyces viridosporus