Application | Comment | Organism |
---|---|---|
biotechnology | to simplify technological processes and reduce production costs, cascade biocatalysis for 2-oxoglutarate production is constructed by simultaneously expressing L-glutamate oxidase (LGOX) from Streptomyces viridosporus and KatG from Escherichia coli W3110 in Escherichia coli BL21 (DE3). In vivo cascade biocatalysis is constructed and optimized by promoter engineering to finely control the coexpression of LGOX and KatG, thus resulting in a significant increase in 2-oxoglutarate concentration and its conversion rate with no catalase addition | Streptomyces viridosporus |
synthesis | to simplify technological processes and reduce production costs, cascade biocatalysis for 2-oxoglutarate production is constructed by simultaneously expressing L-glutamate oxidase (LGOX) from Streptomyces viridosporus and KatG from Escherichia coli W3110 in Escherichia coli BL21 (DE3). In vivo cascade biocatalysis is constructed and optimized by promoter engineering to finely control the coexpression of LGOX and KatG, thus resulting in a significant increase in 2-oxoglutarate concentration and its conversion rate with no catalase addition | Streptomyces viridosporus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces viridosporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.3 | - |
L-glutamate | pH 6.5, 30°C | Streptomyces viridosporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | Streptomyces viridosporus | - |
2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | Streptomyces viridosporus ATCC 14672 | - |
2-oxoglutarate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces viridosporus | D6A5I3 | - |
- |
Streptomyces viridosporus ATCC 14672 | D6A5I3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces viridosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | - |
Streptomyces viridosporus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | - |
Streptomyces viridosporus ATCC 14672 | 2-oxoglutarate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces viridosporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-glutamate | pH 6.5, 30°C | Streptomyces viridosporus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
L-glutamate | pH 6.5, 30°C | Streptomyces viridosporus |