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Literature summary for 1.4.3.12 extracted from

  • Iwaki, H.; Shimizu, M.; Tokuyama, T.; Hasegawa, Y.
    Purification and characterization of a novel cyclohexylamine oxidase from the cyclohexylamine-degrading Brevibacterium oxydans IH-35A (1999), J. Biosci. Bioeng., 88, 264-268.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Quinacrine
-
Microbacterium oxydans
Quinine
-
Microbacterium oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23
-
Cyclohexylamine
-
Microbacterium oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Microbacterium oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexylamine + O2 + H2O Microbacterium oxydans highly specific for alicyclic monoamines and aliphatic monoamines cyclohexanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O Microbacterium oxydans IH-35A highly specific for alicyclic monoamines and aliphatic monoamines cyclohexanone + NH3 + H2O2
-
?
cyclopentylamine + O2 + H2O Microbacterium oxydans highly specific for alicyclic monoamines and aliphatic monoamines cyclopentanone + NH3 + H2O2
-
?
cyclopentylamine + O2 + H2O Microbacterium oxydans IH-35A highly specific for alicyclic monoamines and aliphatic monoamines cyclopentanone + NH3 + H2O2
-
?
N-methylcyclohexylamine + O2 + H2O Microbacterium oxydans highly specific for alicyclic monoamines and aliphatic monoamines cyclohexanone + methylamine + H2O2
-
?
N-methylcyclohexylamine + O2 + H2O Microbacterium oxydans IH-35A highly specific for alicyclic monoamines and aliphatic monoamines cyclohexanone + methylamine + H2O2
-
?
secondary butylamine + O2 + H2O Microbacterium oxydans highly specific for alicyclic monoamines and aliphatic monoamines butanone + NH3 + H2O2
-
?
secondary butylamine + O2 + H2O Microbacterium oxydans IH-35A highly specific for alicyclic monoamines and aliphatic monoamines butanone + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Microbacterium oxydans
-
-
-
Microbacterium oxydans IH-35A
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
Microbacterium oxydans

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Microbacterium oxydans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by growth on cyclohexylamine Microbacterium oxydans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans cyclohexanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans IH-35A cyclohexanone + NH3 + H2O2
-
?
cyclopentylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans cyclopentanone + NH3 + H2O2
-
?
cyclopentylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans IH-35A cyclopentanone + NH3 + H2O2
-
?
N-methylcyclohexylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans cyclohexanone + methylamine + H2O2
-
?
N-methylcyclohexylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans IH-35A cyclohexanone + methylamine + H2O2
-
?
secondary butylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans butanone + NH3 + H2O2
-
?
secondary butylamine + O2 + H2O highly specific for alicyclic monoamines and aliphatic monoamines Microbacterium oxydans IH-35A butanone + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Microbacterium oxydans

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30
-
stable up to Microbacterium oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Microbacterium oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 stable between Microbacterium oxydans