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Literature summary for 1.4.3.12 extracted from

  • Zhou, H.; Han, Z.G.; Fang, T.; Chen, Y.Y.; Ning, S.B.; Gan, Y.T.; Yan, D.Z.
    Characterization of a new cyclohexylamine oxidase from Acinetobacter sp. YT-02 (2018), Front. Microbiol., 9, 2848 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
environmental protection as a potential biocatalyst, the enzyme is promising in controlling cyclohexylamine pollution and deracemization of chiral amines Acinetobacter sp. YT-02

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Acinetobacter sp. YT-02

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 2 mM, 10% loss of activity Acinetobacter sp. YT-02
Co2+ 2 mM, 15% loss of activity Acinetobacter sp. YT-02
K+ 2 mM, 17% loss of activity Acinetobacter sp. YT-02
Mg2+ 2 mM, 28% loss of activity Acinetobacter sp. YT-02

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
Cyclohexylamine pH 7.0, 30┬░C Acinetobacter sp. YT-02
0.25
-
Cyclohexylamine pH 7.0, 50┬░C Acinetobacter sp. YT-02

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
gel filtration Acinetobacter sp. YT-02

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexylamine + O2 + H2O Acinetobacter sp. YT-02
-
cyclohexanone + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. YT-02
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity chromatography Acinetobacter sp. YT-02

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylcyclohexanamine + O2 + H2O 22% of the activity with cyclohexylamine Acinetobacter sp. YT-02 2-methylcyclohexanone + NH3 + H2O2
-
?
4-methylcyclohexanamine + O2 + H2O 41% of the activity with cyclohexylamine Acinetobacter sp. YT-02 4-methylcyclohexanone + NH3 + H2O2
-
?
cycloheptylamine + O2 + H2O 55% of the activity with cyclohexylamine Acinetobacter sp. YT-02 cycloheptanone + NH3 + H2O2
-
?
cyclohexylamine + O2 + H2O
-
Acinetobacter sp. YT-02 cyclohexanone + NH3 + H2O2
-
?
cyclopentylamine + O2 + H2O 7% of the activity with cyclohexylamine Acinetobacter sp. YT-02 cyclopentanone + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer
-
Acinetobacter sp. YT-02

Synonyms

Synonyms Comment Organism
CF596_10820
-
Acinetobacter sp. YT-02
CHAO
-
Acinetobacter sp. YT-02

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Acinetobacter sp. YT-02

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 60 30┬░C: about 70% of maximal activity, 60┬░C: about 70% of maximal activity Acinetobacter sp. YT-02

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
30 min, pH 7.0, more than 40% activity of the enzyme is retained Acinetobacter sp. YT-02
50
-
30 min, activity decreases rapidly when the temperature is higher than 50┬░C Acinetobacter sp. YT-02

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
432
-
Cyclohexylamine pH 7.0, 30┬░C Acinetobacter sp. YT-02
523
-
Cyclohexylamine pH 7.0, 50┬░C Acinetobacter sp. YT-02

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Acinetobacter sp. YT-02

pH Range

pH Minimum pH Maximum Comment Organism
7 9 the enzyme retains more than 60% residual activity at pH 7.0-9.0 Acinetobacter sp. YT-02

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 enzyme activity is maintained at more than 50% level after incubation for 30 min at pH 7.0-9.0 Acinetobacter sp. YT-02

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1724
-
Cyclohexylamine pH 7.0, 30┬░C Acinetobacter sp. YT-02
2075
-
Cyclohexylamine pH 7.0, 50┬░C Acinetobacter sp. YT-02