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Literature summary for 1.4.3.14 extracted from

  • Weber, E.; Tonder, K.; Reinbothe, C.; Unverhau, K.; Weide, H.; Aurich, H.
    L-Lysine alpha-oxidase from Trichoderma viride i4. Purification and characterization (1994), J. Basic Microbiol., 34, 265-276.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,4-diaminobutane 20% inhibition Trichoderma viride
1,5-Diaminopentane 24% inhibition Trichoderma viride
1,6-diaminohexane 43% inhibition Trichoderma viride
6-aminocaproic acid 30% inhibition Trichoderma viride
CoSO4
-
Trichoderma viride
CuCl2
-
Trichoderma viride
HgCl2
-
Trichoderma viride
p-chloromercuribenzoate
-
Trichoderma viride
ZnSO4
-
Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
L-lysine
-
Trichoderma viride
0.625
-
L-ornithine
-
Trichoderma viride
0.68
-
L-arginine
-
Trichoderma viride

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, SDS-PAGE Trichoderma viride
110000
-
gel filtration Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Trichoderma viride preferred substrate 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O Trichoderma viride i4 preferred substrate 6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 60%, complete inactivation after 60 min Trichoderma viride
Ethanol 70%, immediately inactivated Trichoderma viride

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
i4
-
Trichoderma viride i4
-
i4
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, ion-exchange Trichoderma viride

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
Trichoderma viride

Storage Stability

Storage Stability Organism
-20°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, at least 14 months stable without any loss of activity Trichoderma viride
4-37°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, stable for 4-5 days Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + O2 + H2O
-
Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
?
L-arginine + O2 + H2O
-
Trichoderma viride i4 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
?
L-histidine + O2 + H2O only 1% in comparison with L-lysine Trichoderma viride 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O preferred substrate Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O preferred substrate Trichoderma viride i4 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-ornithine + O2 + H2O
-
Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
L-ornithine + O2 + H2O
-
Trichoderma viride i4 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
L-phenylalanine + O2 + H2O 16% in comparison to L-lysine Trichoderma viride phenylpyruvate + NH3 + H2O2
-
?
L-phenylalanine + O2 + H2O 16% in comparison to L-lysine Trichoderma viride i4 phenylpyruvate + NH3 + H2O2
-
?
additional information several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested Trichoderma viride ?
-
?
additional information several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested Trichoderma viride i4 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
stable at relatively high temperatures Trichoderma viride
30
-
30 min, 0.1 M potassium phosphate buffer, reaction rate is between 85% at pH 3.0 and 90% at pH 11.0 Trichoderma viride
70
-
total loss of activity if preincubated at 70°C Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Trichoderma viride

pH Range

pH Minimum pH Maximum Comment Organism
5 11 enzyme activity does not fall below 60%, at pH 3.0 the activity is still 30% Trichoderma viride

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
stable at extreme pH values Trichoderma viride

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per subunit, non-covalently bound Trichoderma viride