Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-diaminobutane | 20% inhibition | Trichoderma viride | |
1,5-Diaminopentane | 24% inhibition | Trichoderma viride | |
1,6-diaminohexane | 43% inhibition | Trichoderma viride | |
6-aminocaproic acid | 30% inhibition | Trichoderma viride | |
CoSO4 | - |
Trichoderma viride | |
CuCl2 | - |
Trichoderma viride | |
HgCl2 | - |
Trichoderma viride | |
p-chloromercuribenzoate | - |
Trichoderma viride | |
ZnSO4 | - |
Trichoderma viride |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
L-lysine | - |
Trichoderma viride | |
0.625 | - |
L-ornithine | - |
Trichoderma viride | |
0.68 | - |
L-arginine | - |
Trichoderma viride |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Trichoderma viride |
110000 | - |
gel filtration | Trichoderma viride |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 + H2O | Trichoderma viride | preferred substrate | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | Trichoderma viride i4 | preferred substrate | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 60%, complete inactivation after 60 min | Trichoderma viride |
Ethanol | 70%, immediately inactivated | Trichoderma viride |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma viride | - |
i4 | - |
Trichoderma viride i4 | - |
i4 | - |
Purification (Comment) | Organism |
---|---|
acetone precipitation, ion-exchange | Trichoderma viride |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Trichoderma viride |
Storage Stability | Organism |
---|---|
-20°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, at least 14 months stable without any loss of activity | Trichoderma viride |
4-37°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, stable for 4-5 days | Trichoderma viride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + O2 + H2O | - |
Trichoderma viride | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-arginine + O2 + H2O | - |
Trichoderma viride i4 | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-histidine + O2 + H2O | only 1% in comparison with L-lysine | Trichoderma viride | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | preferred substrate | Trichoderma viride | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | preferred substrate | Trichoderma viride i4 | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | - |
Trichoderma viride | 5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | - |
Trichoderma viride i4 | 5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-phenylalanine + O2 + H2O | 16% in comparison to L-lysine | Trichoderma viride | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylalanine + O2 + H2O | 16% in comparison to L-lysine | Trichoderma viride i4 | phenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested | Trichoderma viride | ? | - |
? | |
additional information | several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested | Trichoderma viride i4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Trichoderma viride |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma viride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
stable at relatively high temperatures | Trichoderma viride |
30 | - |
30 min, 0.1 M potassium phosphate buffer, reaction rate is between 85% at pH 3.0 and 90% at pH 11.0 | Trichoderma viride |
70 | - |
total loss of activity if preincubated at 70°C | Trichoderma viride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Trichoderma viride |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | enzyme activity does not fall below 60%, at pH 3.0 the activity is still 30% | Trichoderma viride |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
stable at extreme pH values | Trichoderma viride |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per subunit, non-covalently bound | Trichoderma viride |