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Literature summary for 1.4.3.14 extracted from

  • Chen, W.; Lin, C.; Sheu, S.
    Investigating antimicrobial activity in Rheinheimera sp. due to hydrogen peroxide generated by L-lysine oxidase activity (2010), Enzyme Microb. Technol., 46, 487-493.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons, the enzyme shows 100% sequence identitiy with gene alpP of Pseudoalteromonas tunicata D2 encoding an autolytic and antibacterial protein Rheinheimera sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
native PAGE Rheinheimera sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Rheinheimera sp.
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O Rheinheimera sp. GR5
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rheinheimera sp.
-
Phylogenetic tree based on 16S rRNA gene sequences, overview
-
Rheinheimera sp. GR5
-
Phylogenetic tree based on 16S rRNA gene sequences, overview
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
Rheinheimera sp. 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O
-
Rheinheimera sp. GR5 6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Rheinheimera sp.

pI Value

Organism Comment pI Value Maximum pI Value
Rheinheimera sp. isoelectric focusing
-
3.68

General Information

General Information Comment Organism
additional information antimicrobial activity in Rheinheimera sp. due to hydrogen peroxide generated by L-lysine oxidase activity against Gram-positive and Gram-negative bacteria, yeast, algae, and strain GR5 itself, overview. This antimicrobial effect is active only in complex culture media or chemically defined culture media containing L-lysine and can be inhibited by catalase Rheinheimera sp.