Any feedback?
Please rate this page

BRENDA support

Literature summary for extracted from

  • Sakuraba, H.; Yoneda, K.; Asai, I.; Tsuge, H.; Katunuma, N.; Ohshima, T.
    Structure of L-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii (2008), Biochim. Biophys. Acta, 1784, 563-571.
    View publication on PubMed


Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) codon plus RIL Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 23% (w/v) polyethylene glycol 8000, 0.2 M MgCl2 and 0.1 M Tris/HCl buffer (pH 8.6) Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
L-aspartate recombinant enzyme, at 37°C Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
gel filtration Sulfurisphaera tokodaii
recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii
calculated from the amino acid sequence Sulfurisphaera tokodaii


Organism UniProt Comment Textmining
Sulfurisphaera tokodaii

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Butyl Toyopearl column chromatography Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
the specific activity of the purified enzyme is estimated to be 0.7 units/mg at 37°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + H2O + O2
Sulfurisphaera tokodaii oxaloacetate + NH3 + H2O2


Subunits Comment Organism
monomer 1 * 47000, gel filtration Sulfurisphaera tokodaii


Synonyms Comment Organism
Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
the enzyme retains more than 90% of its activity when heated for 10 min at 80°C, the enzyme retains 70% of its activity after incubation for 1 h at 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
around pH 8.0 Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.7 10.3 when heated at 50 °C for 30 min, the enzyme does not lose activity at pH values from 3.7 to 10.3 Sulfurisphaera tokodaii


Cofactor Comment Organism Structure
Sulfurisphaera tokodaii